Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
Immunoprecipitation (IP) | 3 µg |
Western Blot (WB) | 0.25-2 µg/mL |
Immunocytochemistry (ICC/IF) | 15 -25 µg/mL |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 2 publications below |
Published species | Human, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic phosphopeptide corresponding to residues R(82) Q L S(p) S G V S E I R(92) C of rat HSP27. |
Conjugate | Unconjugated |
Form | Lyophilized |
Concentration | 1 mg/mL |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_325061 |
PA1-005 detects phosphorylated heat shock protein 27 (HSP27) from human and rat tissue samples. This antibody does not detect the unphosphorylated form of the protein.
PA1-005 has been successfully used in Western blot, immunoprecipitation, and immunofluorescence procedures. By Western blot, this antibody detects an ~27 kDa protein representing phosphorylated HSP27(Ser85) from rat skeletal muscle extracts.
The PA1-005 immunogen is a synthetic phosphopeptide corresponding to residues R(82) Q L S(p) S G V S E I R(92) C of rat HSP27. This sequence is completely conserved in mouse.
Reconstitute with 100 µL for a 1 mg/mL solution.
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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