R&D Systems

Manufacturer Code:MAB5870SP

PU.1/Spi-1 Mouse anti-Human, Clone: 732322, R&D Systems™

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Detection of Human PU.1/Spi-1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 g/mL of Mouse Anti-Human PU.1/Spi-1 Monoclonal Antibody (Catalog # MAB5870) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PU.1/Spi-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of PU.1/Spi-1 in THP-1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human PU.1/Spi-1 Monoclonal Antibody (Catalog # MAB5870, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Product Details

TESTED APPLICATIONS	DILUTION
Flow Cytometry (Flow)	Assay dependent
Western Blot (WB)	Assay dependent
CyTOF (CyTOF)	Assay dependent

Species reactivity	Human
Host / Isotype	Mouse
Class	Monoclonal
Type	Antibody
Clone	732322
Immunogen	<EM>E. coli</EM>-derived recombinant human PU.1/Spi-1 Met1-Lys169 Accession # NP_001074016
Conjugate	Unconjugated
Form	Liquid
Purification	purified
Storage buffer	PBS, pH7.4
Contains	15mM sodium azide
Storage conditions	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt -20 to -70 degreesC as supplied. 1 month 2 to 8 degreesC under sterile conditions after reconstitution. 6 months -20 to -70 degreesC under sterile con

Product Specific Information

Mouse Monoclonal Antibody

For Research Use Only

Bioinformatics

Protein Aliases: 31 kDa transforming protein; 31 kDa-transforming protein; hematopoietic transcription factor PU.1; hematopoietic transcription factor PU.1 OF PU.1 SFPI1 SFPI131 kDa transforming protein SPI1 SPI-1 SPI-1 proto-oncogene SPI-A spleen focus forming virus (SFFV) proviral integration oncogene spi131 kDa-transforming protein transcription factor PU.1; spleen focus forming virus (SFFV) proviral integration oncogene spi1; Transcription factor PU.1

Gene Aliases: OF; PU.1; SFPI1; SPI-1; SPI-A; SPI1

UniProt ID: (Human) P17947

Entrez Gene ID: (Human) 6688

Molecular Function: Blue Arrow Icon nucleic acid binding signaling molecule transcription factor

Function(s)

negative regulation of transcription from RNA polymerase II promoter lymphoid progenitor cell differentiation transcription from RNA polymerase II promoter lymphocyte differentiation erythrocyte differentiation macrophage differentiation granulocyte differentiation somatic stem cell population maintenance myeloid dendritic cell differentiation histone H3 acetylation hypermethylation of CpG island negative regulation of MHC class II biosynthetic process regulation of erythrocyte differentiation negative regulation of gene expression, epigenetic negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter anatomical structure regression cellular response to ethanol negative regulation of histone H4 acetylation apoptotic process involved in patterning of blood vessels positive regulation of pri-miRNA transcription from RNA polymerase II promoter

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
RNA binding
protein binding
NFAT protein binding

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PU.1/Spi-1 Mouse anti-Human, Clone: 732322, R&D Systems™

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PU.1/Spi-1 Mouse anti-Human, Clone: 732322, R&D Systems™

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Product Details

Product Specific Information

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

Performance Guarantee

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