Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA5-49786
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Western Blot (WB) | 1:500-1:3,000 |
Immunocytochemistry (ICC/IF) | 5 µg/ml |
Species reactivity | Human, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthesized peptide derived from N-terminal of human KAP0. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS, pH 7.4, with 50% glycerol |
Contains | 0.02% sodium azide |
Storage conditions | -20°C |
RRID | AB_2635239 |
The antibody detects endogenous levels of total PKA R1 alpha protein.
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cAMP-dependent protein kinase regulatory subunit RIalpha; cAMP-dependent protein kinase type I-alpha regulatory chain; cAMP-dependent protein kinase type I-alpha regulatory subunit; Carney complex type 1; protein kinase A type 1a regulatory subunit; protein kinase, cAMP dependent regulatory, type 1, alpha; protein kinase, cAMP dependent regulatory, type I, alpha; protein kinase, cAMP-dependent, regulatory subunit type I alpha; protein kinase, cAMP-dependent, regulatory, type I, alpha; Tissue-specific extinguisher 1; TSE1
Gene Aliases: 1300018C22Rik; ACRDYS1; ADOHR; CAR; CNC; CNC1; PKR1; PPNAD1; PRKAR1; PRKAR1A; RIalpha; RIIA; Tse-1; TSE1
UniProt ID: (Human) P10644, (Rat) P09456, (Mouse) Q9DBC7
Entrez Gene ID: (Human) 5573, (Rat) 25725, (Mouse) 19084
Molecular Function:
cytoskeletal protein
protein-binding activity modulator
kinase modulator
microtubule or microtubule-binding cytoskeletal protein
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