Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
TESTED APPLICATIONS | DILUTION |
---|---|
ELISA (ELISA) | Assay-dependent |
Western Blot (WB) | 0.5 µg/mL |
Immunocytochemistry (ICC/IF) | 2 µg/mL |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Clone | 13H5 |
Immunogen | Recombinant human protein purified from E.coli (His-PRAK) |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2141477 |
A suggested positive control for this product is A431 cells.
MAPKAPK5 (PRAK) is activated in response to cellular stress and pro-inflammatory cytokines. Activity of this kinase is regulated by p38 alpha and p38 beta. The protein encoded by PRAK is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of PRAK encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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