Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA1-650
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Flow Cytometry (Flow) | 0.5 µg/test |
Western Blot (WB) | 1:500 |
Immunocytochemistry (ICC/IF) | 1-10 µg/mL |
PUBLISHED APPLICATIONS | |
---|---|
Immunocytochemistry (ICC/IF) | See 11 publications below |
Immunohistochemistry (IHC) | See 3 publications below |
Immunohistochemistry (Paraffin) (IHC (P)) | See 1 publications below |
Western Blot (WB) | See 10 publications below |
Miscellaneous PubMed (Misc) | See 1 publications below |
Immunoprecipitation (IP) | See 1 publications below |
Flow Cytometry (Flow) | See 2 publications below |
Species reactivity | Human, Mouse, Rat |
Published species | Hamster, Human, Mouse, Non-human primate, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic Peptide: C N(644) Y E F K K I T E D T V E F G S(659) |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2219912 |
PA1-650 detects peroxisomal membrane protein 70 (PMP70) from mouse tissues.
PA1-650 has been successfully used in Western blot, flow cytometry, and immunofluorescence procedures. By Western blot, this antibody detects an ~70 kDa protein representing PMP70 from peroxisome-enriched fractions from L cells.
PA1-650 immunizing peptide corresponds to amino acid residues 644-659 from rat PMP70. This sequence is completely conserved in mouse PMP70 and differs from human PMP70 by a single amino acid substitution. PA1-650 immunizing peptide (Cat. # PEP-038) is available for use in neutralization and control experiments.
Peroxisomes are single membrane organelles which are involved with many important biochemical pathways and are found in almost all eukaryotic cells. Various toxins including, phenols, alcohols, and aldehydes are targeted for modification by peroxisomes. Long chain fatty acid metabolism via beta-oxidation is also mediated by this organelle. Loss of peroxisomal functions may result in diseases such as Zellweger syndrome, hyperpipecolic acidemia, neonatal adrenoleukodystrophy, and infantile Refsum" disease. Peroxisomal membrane protein 70 (PMP70) is an abundant, integral membrane protein of the peroxisome. This protein is induced by treatment with hypolipidemic agents in parallel with peroxisome proliferation and stimulation of the peroxisomal beta-oxidation enzymes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 68 kDa peroxisomal membrane protein; 70 kDa peroxisomal membrane protein; 70-kDa peroxisomal membrane protein; ATP-binding cassette sub-family D member 3; ATP-binding cassette, sub-family D (ALD), member 3; dJ824O18.1 (ATP-binding cassette, sub-family D (ALD), member 3 (PMP70, PXMP1)); Peroxisomal membrane protein 1; peroxisomal membrane protein 1 (70kD, Zellweger syndrome); peroxisomal membrane protein, 70 kDa; Peroxisomal membrane protein-1 (70kD); PMP68; PMP70
Gene Aliases: ABC43; ABCD3; AI313901; AU018866; AW146054; CBAS5; PMP68; PMP70; PXMP1; ZWS2
UniProt ID: (Human) P28288, (Mouse) P55096, (Rat) P16970
Entrez Gene ID: (Human) 5825, (Mouse) 19299, (Rat) 25270
Molecular Function:
ATP-binding cassette (ABC) transporter
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