Invitrogen
Catalog # PA5-50346
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Paraffin) (IHC (P)) | 1:25-1:100 |
Western Blot (WB) | 1:200-1:1,000 |
Species reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Fusion protein corresponding to residues near the C terminal of human phosphatidylinositol-4-phosphate 5-kinase, type I, alpha |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1.3 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS, pH 7.4, with 40% glycerol |
Contains | 0.05% sodium azide |
Storage conditions | -20°C |
RRID | AB_2635799 |
The antibody detects endogenous levels of total PIP5K1A protein.
PIP5K1A lipid kinase or phosphatidylinositol-4-phosphate 5-kinase 1 alpha phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1 is required for Wnt3a-induced LRP6 phosphorylation at ser1490 in mammalian cells. Wnt3a stimulates the formation of phosphatidylinositol 4,5-bisphosphates through 'frizzled' and 'disheveled', the latter of which directly interacts with and activated PIP5KI. PIP5K1A-mediated PIP(2) production is crucial for HIV-1 entry and the early steps of infection in permissive lymphocytes. Overexpression of wild-type PI4P5K1A increases HIV-1 Env-mediated PIP(2) production and enhances viral replication in primary lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha; 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha; Phosphatidylinositol 4-phosphate 5-kinase type I alpha; Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha; phosphatidylinositol-4-phosphate 5-kinase; phosphatidylinositol-4-phosphate 5-kinase type-1 alpha; phosphatidylinositol-4-phosphate 5-kinase, type I, alpha; PI51A; PIP5K1-alpha; PIP5KIalpha; ptdIns(4)P-5-kinase 1 alpha
Gene Aliases: PIP5K1A
UniProt ID: (Human) Q99755
Entrez Gene ID: (Human) 8394
Molecular Function:
kinase
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