Invitrogen
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. View details.
Catalog # MA1-26528
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
ELISA (ELISA) | Assay-dependent |
Flow Cytometry (Flow) | 5-10 µg for 1x10^6 cells |
Immunohistochemistry (Frozen) (IHC (F)) | 5-10 µg/mL |
Immunohistochemistry (Paraffin) (IHC (P)) | 1:25 |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1:50-1:600 |
Immunocytochemistry (ICC/IF) | Assay-dependent |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 22 publications below |
Immunohistochemistry (IHC) | See 5 publications below |
Immunocytochemistry (ICC/IF) | See 3 publications below |
Species reactivity | Bovine, Fish, Hamster, Human, Mouse, Rat, Zebrafish |
Published species | Dog, Fish, Human, Mouse, Rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Clone | C219 |
Immunogen | SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 0.72 mg/mL |
Purification | purified |
Storage buffer | PBS with 1% BSA |
Contains | 0.1% sodium azide |
Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID | AB_795165 |
Recommended positive controls: Cell lines or frozen tissue, such as liver or colon.
MA1-26528 is expected to cross react with a wide range of mammals due to sequence homology.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
This clone recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, MDR1 P-glycoprotein (~170kDa) and MDR3 P-glycoprotein (~140kDa). In Western blot, boiling samples may reduce visibility of the protein.
For flow cytometry, cells should be fixed in 3.7% formaldehyde for 10 min at room temperature to permeabilize the cell membrane. Incubate cells with 5-10 µg antibody for 30-60 min at 4ºC.
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ATP-binding cassette sub-family B (MDR/TAP) member 1 (P-glycoprotein/multidrug resistance 1); ATP-binding cassette sub-family B member 1; ATP-binding cassette sub-family B member 1A; ATP-binding cassette sub-family B member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1 (P-glycoprotein/multidrug resistance 1); ATP-binding cassette, sub-family B (MDR/TAP), member 1A; ATP-binding cassette, sub-family B (MDR/TAP), member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 4; ATP-binding cassette, subfamily B (MDR/TAP), member 1A; ATP-binding cassette, subfamily B, member 1A; ATP-binding cassette, subfamily B, member 1B; ATP-dependent translocase ABCB1; CD243; colchicin sensitivity; doxorubicin resistance; ecotropic viral integration site 32; MDR1A; multi-drug resistance 3; Multidrug resistance protein 1; Multidrug resistance protein 1A; Multidrug resistance protein 1B; Multidrug resistance protein 2; Multidrug resistance protein 3; multiple drug resistant 1a; P glycoprotein 1; P glycoprotein 3; P-glycoprotein; P-glycoprotein 1; P-glycoprotein 2; P-glycoprotein 3; P-glycoprotein/multidrug resistance 1; Phospholipid transporter ABCB1
Gene Aliases: ABC20; ABCB1; Abcb1a; Abcb1b; Abcb4; CD243; CLCS; Evi32; GP170; mdr; mdr-3; MDR1; Mdr1a; Mdr1b; Mdr3; P-GP; Pgp; PGP1; PGP2; Pgy-1; Pgy-3; PGY1; Pgy1-1; PGY2; Pgy3
UniProt ID: (Human) P08183, (Mouse) P21447, (Chinese hamster) P21448, (Mouse) P06795, (Chinese hamster) P21449
Entrez Gene ID: (Human) 5243, (Bovine) 281585, (Mouse) 18671, (Rat) 170913, (Chinese hamster) 100682536, (Mouse) 18669, (Rat) 24646, (Chinese hamster) 100682537
Molecular Function:
ATP-binding cassette (ABC) transporter
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