Catalog # PA1-780
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Western Blot (WB) | 1-4 µg/mL |
Immunocytochemistry (ICC/IF) | 10 µg/mL |
PUBLISHED APPLICATIONS | |
---|---|
Immunohistochemistry (Paraffin) (IHC (P)) | See 2 publications below |
Immunocytochemistry (ICC/IF) | See 11 publications below |
Western Blot (WB) | See 4 publications below |
Immunohistochemistry (IHC) | See 16 publications below |
Immunomicroscopy (IM) | See 1 publications below |
Miscellaneous PubMed (Misc) | See 1 publications below |
Species reactivity | Mouse, Rat |
Published species | Fruit fly, Hamster, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptide corresponding to residues K M(1) N S P S E S R V P S S L T Q D P S F(19) of rat melanopsin. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2267547 |
PA1-780 detects mouse and rat melanopsin.
PA1-780 has been successfully used in Western blot, immunofluorescence, and immunocytochemistry procedures. By Western blot, this antibody detects ~53 and ~85 kDa proteins representing overexpressed unglycosylated and glycosylated melanopsin from HEK 293 cells (does not work well on endogenously expressed protein). Immunofluorescence and immunocytochemistry staining of melanopsin in rat retinal ganglion cells (RGCs) can be performed using PA1-780.
PA1-780 immunogen is a synthetic peptide corresponding to residues K M(1) N S P S E S R V P S S L T Q D P S F(19) of rat melanopsin.
Melanopsin is an opsin-like protein found in the retinal ganglion cells of mammals. Melanopsin is believed to be part of a secondary optical system that parallels that of the classic rod and cone system. This second system reports directly to the suprachiasmatic nucleus and is responsible for regulation of circadian rhythms. Melanopsin is believed to be the primary photopigment responsible for the regulation of these circadian rhythms, and Melanopsin knockout mice have been generated which demonstrate decreased capacity to entrain to light and dark cycles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: LOC721663; Melanopsin; MGC142118; OPN 4; opn4 {ECO:0000312|RGD:621701}; Opsin-4
Gene Aliases: 1110007J02Rik; Gm533; Mop; Mopn; Opn4
UniProt ID: (Mouse) Q9QXZ9, (Rat) Q8R456
Entrez Gene ID: (Mouse) 30044, (Rat) 192223
Molecular Function:
G-protein coupled receptor
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