Invitrogen

Manufacturer Code:PA116919

Nucleolin Rabbit anti-Human, Rat, Polyclonal, Invitrogen™

View (86) other Nucleolin antibodies

Data Sheets

Create Citation

Tech Support

Catalog # 01-950-161

Check your price

Quantity:

Request bulk or custom quote

Add To Cart

Performance Guarantee

Product Image Gallery

Nucleolin Rabbit [PA116919] — Detection of Nucleolin (green) in Hela cells detected with a Dylight-488 conjugated secondary antibody. Nuclei are counter stained with Hoechst 33258.

Nucleolin Antibody (PA1-16919) in IF — Immunofluorescence analysis of Nucleolin was performed using 70% confluent log phase HeLa cells treated with Heat Shock (44 degrees for 3 hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Nucleolin Polyclonal Antibody (Product ## PA1-16919) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat

Nucleolin Antibody (PA1-16919) in KD — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Nucleolin siRNA and reduction of signal was observed in Western Blot using Nucleolin Polyclonal Antibody (Product ## PA1-16919).

Nucleolin Antibody (PA1-16919) in WB — Western blot analysis was performed on modified whole cell extracts (30 µg lysate) of HeLa (Lane 1), U-2 OS (Lane 2), NTERA-2 (Lane 3) and Raji (Lane 4). The blot was probed with Anti-Nucleolin Polyclonal Antibody (Product ## PA1-16919, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product ## A27036, 0.25 µg/ml, 1:4000 dilution). A 100 kDa band corresponding to Nucleolin was observed across th

Product Details

TESTED APPLICATIONS	DILUTION
Immunocytochemistry (ICC)	Assay dependent
Immunofluorescence (IF)	Assay dependent
Immunohistochemistry (Paraffin) (IHC (P))	Assay dependent
Immunoprecipitation (IP)	Assay dependent
Western Blot (WB)	Assay dependent

Species reactivity	Many
Host / Isotype	Rabbit
Class	Polyclonal
Type	Antibody
Immunogen	15 residue peptide designed to C-terminus of the human Nucleolin protein
Conjugate	Unconjugated
Form	Liquid
Purification	purified
Storage buffer	PBS, pH7.4
Contains	15mM sodium azide
Storage conditions	-20&deg C Avoid Freeze/Thaw Cycles

Product Specific Information

Rabbit Polyclonal Antibody

For Research Use Only

Bioinformatics

Protein Aliases: nucl antibody protein C23 antibody.; Nucleolin; nucleolin-like protein; Protein C23

Gene Aliases: C23; NCL

UniProt ID: (Human) P19338

Entrez Gene ID: (Human) 4691, (Rat) 25135

Molecular Function: Blue Arrow Icon ribosomal protein

Function(s)

angiogenesis endocytosis spermatogenesis positive regulation of tumor necrosis factor production negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter cellular response to lipopolysaccharide liver regeneration positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter regulation of rRNA processing positive regulation of interleukin-6 secretion

Process(es)

nucleotide binding
single-stranded DNA binding
RNA binding
calcium ion binding
protein binding
protein C-terminus binding
selenocysteine insertion sequence binding
rRNA primary transcript binding
telomeric DNA binding
histone binding
identical protein binding
laminin binding
poly(A) RNA binding
ErbB-4 class receptor binding
receptor binding
sequence-specific DNA binding

Suggested Secondary Antibodies

Anti-Rabbit Secondary Antibodies (730)

Alexa Fluor® Anti-Rabbit Secondary Antibodies (91)

HRP Anti-Rabbit Secondary Antibodies (100)

Documents

Material safety data sheets (MSDS)

Request a MSDS

Performance Guarantee

If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Nucleolin Rabbit anti-Human, Rat, Polyclonal, Invitrogen™

Product Details

Product Specific Information

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

Performance Guarantee

We’re here to help

Your items have has been added!

Nucleolin Rabbit anti-Human, Rat, Polyclonal, Invitrogen™

×

Product Details

Product Specific Information

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

Performance Guarantee

We’re here to help

Your items have has been added!