Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA1-023
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Gel Shift (GS) | Assay-dependent |
Immunohistochemistry (Paraffin) (IHC (P)) | 1:20 |
Immunoprecipitation (IP) | 3 µg |
Western Blot (WB) | 1:1,000 |
Immunocytochemistry (ICC/IF) | 1:1,000 |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 24 publications below |
Immunocytochemistry (ICC/IF) | See 1 publications below |
ChIP assay (ChIP) | See 2 publications below |
Immunohistochemistry (IHC) | See 3 publications below |
Species reactivity | Human, Mouse |
Published species | Human, Mouse, Rabbit, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2152617 |
PA1-023 detects nuclear factor of activated T-cells 5 (NFAT5) from human and mouse cells as well as recombinant human protein.
PA1-023 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, gel shift and immunocytochemistry procedures. By Western blot, this antibody detects an ~170 kDa protein representing NFAT5 in HEK293 cells transfected with the human NFAT5 gene. Immunocytochemical staining of NFAT5 in HEK293 cells transfected with the human NFAT5 gene with PA1-023 results in primarily cytoplasmic staining.
The PA1-023 immunogen is a synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5. This sequence is 94% conserved in the mouse protein. PA1-023 immunizing peptide (Cat. # PEP-123) is available for use in neutralization and control experiments.
The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: glutamine rich protein H65; NF-AT5; NFAT-like protein 1; Nuclear factor of activated T-cells 5; osmotic response element-binding protein; Rel domain-containing transcription factor NFAT5; T-cell transcription factor NFAT5; TonE-binding protein; tonicity-responsive enhancer binding protein; Tonicity-responsive enhancer-binding protein
Gene Aliases: AI225870; B130038B15Rik; CAG-8; CAG80; KIAA0827; mKIAA0827; NF-AT5; NFAT5; NFATL1; NFATZ; OREBP; TONEBP
UniProt ID: (Human) O94916, (Mouse) Q9WV30
Entrez Gene ID: (Human) 10725, (Mouse) 54446
Molecular Function:
Rel homology transcription factor
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