Invitrogen
Catalog # PA5-49726
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
ELISA (ELISA) | 1:2,000-1:5,000 |
Immunohistochemistry (Paraffin) (IHC (P)) | 1:25-1:100 |
Western Blot (WB) | 1:500-1:2,000 |
Species reactivity | Human, Mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptide peptide corresponding to a region derived from 94-107 amino acids of human NCK adaptor protein 2 |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1.8 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS, pH 7.4, with 40% glycerol |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2635179 |
The antibody detects endogenous level of total NCK2 protein.
The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 and NCK2, which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cytoplasmic protein NCK2; Growth factor receptor-bound protein 4; NCK adaptor protein 2; Nck-2; noncatalytic region of tyrosine kinase, beta; SH2/SH3 adaptor protein NCK-beta
Gene Aliases: 4833426I10Rik; GRB4; Nck-2; NCK2; NCKbeta
UniProt ID: (Human) O43639, (Mouse) O55033
Entrez Gene ID: (Human) 8440, (Mouse) 17974
Molecular Function:
scaffold/adaptor protein
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