Catalog # MA3-942
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
ELISA (ELISA) | Assay-dependent |
Flow Cytometry (Flow) | Assay-dependent |
Immunohistochemistry (Frozen) (IHC (F)) | 1:50 |
Western Blot (WB) | 1:500 |
Immunocytochemistry (ICC/IF) | 1:20 |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 8 publications below |
Immunohistochemistry (IHC) | See 1 publications below |
Species reactivity | Bovine, Human, Pig, Rabbit, Rat |
Published species | Bovine, Fish, Human, Pig, Rabbit, Yeast |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Clone | 107-82 |
Immunogen | Purified bovine skeletal muscle 80 kDa subunit of m-calpain. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Protein A |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2290793 |
MA3-942 detects the 80 kDa subunit of m-calpain in human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, pig cultured cells, and rabbit samples. This antibody does not cross-react with mu-calpain, n-calpain, calmodulin or calpastatin.
MA3-942 has been successfully used in Western blot, IF, immunohistochemistry, and ELISA procedures. By Western blot, this antibody detects an ~80 kDa protein representing m-calpain in bovine platelets. Immunocytochemical staining of m-calpain in LLC-PK1 cells with MA3-942 results in diffuse cytoplasmic staining. This product has not been shown to be effective in immunoprecipitation experiments.
The MA3-942 antigen is purified bovine skeletal muscle 80 kDa subunit of m-calpain. This antibody recognizes an epitope between amino acids 502-699 (domain III/IV) of human calpain.
Mu-Calpain, also known as Calpain-I, and m-calpain, also known as Calpain-II, are intracellular, calcium-dependent cysteine proteases. Mu-calpain has a micromolar sensitivity (thus the mu-) as compared to the millimolar calcium sensitivity of m-calpain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Calcium-activated neutral proteinase 2; calcium-dependent protease; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; calpain II; Calpain large polypeptide L2; Calpain M-type; calpain, large polypeptide L2; Calpain-2 catalytic subunit; Calpain-2 large subunit; CANP 2; M-calpain; Millimolar-calpain
Gene Aliases: CANP 2; CANP2; CANPL2; CANPml; CAPN2; mCANP
UniProt ID: (Bovine) Q27971, (Human) Q8WU26, (Rat) Q07009
Entrez Gene ID: (Rabbit) 100009092, (Pig) 397393, (Bovine) 281662, (Human) 824, (Rat) 29154
Molecular Function:
cysteine protease
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