Invitrogen

ME2 Polyclonal Antibody

View (17) other ME2 antibodies

Data Sheets

Create Citation

Tech Support

Catalog # PA5-48407

Check your price

Quantity:

Request bulk or custom quote

Add To Cart

Performance Guarantee

Product Image Gallery

ME2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ME2 in formalin fixed and paraffin embedded human rectum tissue. Samples were incubated with ME2 polyclonal antibody (Product # PA5-48407) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

ME2 Antibody in Western Blot (WB) — Western blot analysis of ME2 in mouse NIH-3T3, Neuro-2a cell line lysates. Samples were incubated with ME2 polyclonal antibody (Product # PA5-48407). Lysates: 35 µg/lane. ME2 protein (arrow).

Product Details

TESTED APPLICATIONS	DILUTION
Immunohistochemistry (Paraffin) (IHC (P))	1:10-1:50
Western Blot (WB)	1:1,000

Species reactivity	Human, Mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide between 527-556 amino acids from the C-terminal region of human ME2.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A, Antigen affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2633864

Background/Target Information

ME2 encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: malate dehydrogenase; malate dehydrogenase (oxaloacetate-decarboxylating); Malic enzyme 2; malic enzyme 2, NAD(+)-dependent, mitochondrial; NAD-dependent malic enzyme, mitochondrial; NAD-ME; pyruvic-malic carboxylase

Gene Aliases: AW120568; D030040L20Rik; ME2; NAD-ME; ODS1

UniProt ID: (Human) P23368, (Mouse) Q99KE1

Entrez Gene ID: (Human) 4200, (Mouse) 107029

Molecular Function: Blue Arrow Icon oxidoreductase

Function(s)

malate metabolic process oxidation-reduction process regulation of NADP metabolic process metabolic process

Process(es)

malic enzyme activity
malate dehydrogenase (decarboxylating) (NAD+) activity
oxaloacetate decarboxylase activity
electron carrier activity
metal ion binding
NAD binding
catalytic activity
oxidoreductase activity

Suggested Secondary Antibodies

Anti-Rabbit Secondary Antibodies (730)

Alexa Fluor® Anti-Rabbit Secondary Antibodies (91)

HRP Anti-Rabbit Secondary Antibodies (100)

Documents

Material safety data sheets (MSDS)

Request a MSDS

Performance Guarantee

If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*

ME2 Polyclonal Antibody

Product Details

Background/Target Information

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

Performance Guarantee

We’re here to help

Your items have has been added!

ME2 Polyclonal Antibody

×

Product Details

Background/Target Information

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

Performance Guarantee

We’re here to help

Your items have has been added!