Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View details.
Catalog # MA5-23524
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Frozen) (IHC (F)) | 1:100-1:1,000 |
Immunohistochemistry (Paraffin) (IHC (P)) | 1:100-1:1,000 |
Western Blot (WB) | 1:500-1:3,000 |
Species reactivity | Human, Mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Clone | 3I6 |
Immunogen | Recombinant protein encompassing a sequence within the center region of human ME1. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Protein A |
Storage buffer | PBS |
Contains | no preservative |
Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID | AB_2608706 |
Recommended positive controls: 293T, A431, and HeLa cells.
Predicted reactivity: Mouse (91%), Rat (91%), Chicken (80%), Sheep (92%), Bovine (91%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
ME1 encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: malate dehydrogenase; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)); Malic enzyme 1; malic enzyme 1, NADP(+)-dependent, cytosolic; malic enzyme 1, soluble; Malic enzyme, cytoplasmic; malic enzyme, supernatant; NADP-dependent malic enzyme; NADP-ME; pyruvic-malic carboxylase
Gene Aliases: D9Ertd267e; HUMNDME; Mdh-1; ME1; MES; Mod-1; Mod1
UniProt ID: (Human) P48163, (Mouse) P06801
Entrez Gene ID: (Human) 4199, (Mouse) 17436
Molecular Function:
oxidoreductase
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