R&D Systems

Manufacturer Code:MAB7049

Isocitrate Dehydrogenase 1/IDH1 Mouse anti-Human, Clone: 843219, R&D Systems™

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Isocitrate Dehydrogenase 1/IDH1 in SK-BR-3 Human Cell Line. Isocitrate Dehydrogenase 1/IDH1 was detected in immersion fixed SK-BR-3 human breast cancer cell line using Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) at 10 g/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Isocitrate Dehydrogenase 1/IDH1 in Rat Brain. Isocitrate Dehydrogenase 1/IDH1 was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) at 15 g/mL overnight at 4 C. Tissue was stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to glial cell cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Isocitrate Dehydrogenase 1/IDH1 in Human Brain. Isocitrate Dehydrogenase 1/IDH1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) at 15 g/mL overnight at 4 C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to astrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human, Mouse, and Rat Isocitrate Dehydrogenase 1/IDH1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.25 g/mL of Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Isocitrate Dehydrogenase 1/IDH1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Shows Human Isocitrate Dehydrogenase 1/IDH1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Isocitrate Dehydrogenase 1/IDH1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected

Product Details

TESTED APPLICATIONS	DILUTION
Immunocytochemistry (ICC)	Assay dependent
Immunohistochemistry (IHC)	Assay dependent
Western Blot (WB)	Assay dependent

Species reactivity	Human
Host / Isotype	Mouse
Class	Monoclonal
Type	Antibody
Clone	843219
Immunogen	<EM>E. coli</EM>-derived recombinant human Isocitrate Dehydrogenase 1/IDH1 Ser2-Leu414 Accession # O75874
Conjugate	Unconjugated
Form	Liquid
Purification	purified
Storage buffer	PBS, pH7.4
Contains	15mM sodium azide
Storage conditions	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt -20 to -70 degreesC as supplied. 1 month 2 to 8 degreesC under sterile conditions after reconstitution. 6 months -20 to -70 degreesC under sterile con

Product Specific Information

Mouse Monoclonal Antibody

For Research Use Only

Bioinformatics

Protein Aliases: Cytosolic NADP-isocitrate dehydrogenase; Cytosolic NADP-isocitrate dehydrogenase EC 1.1.1.42 IDCD IDH IDH1 IDP IDPC isocitrate dehydrogenase [NADP] cytoplasmic isocitrate dehydrogenase 1 (NADP+) soluble NADP(+)-specific ICDH NADP-dependent isocitrate dehydrogenase cytosolic NADP-dependent isocitrate dehydrogenase peroxisomal Oxalosuccinate decarboxylase PICD; epididymis luminal protein 216; epididymis secretory protein Li 26; IDH; IDP; isocitrate dehydrogenase 1 (NADP+); isocitrate dehydrogenase 1 (NADP+), soluble; Isocitrate dehydrogenase [NADP] cytoplasmic; NADP(+)-specific ICDH; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; Oxalosuccinate decarboxylase

Gene Aliases: HEL-216; HEL-S-26; IDCD; IDH; IDH1; IDP; IDPC; PICD

UniProt ID: (Human) O75874

Entrez Gene ID: (Human) 3417

Function(s)

glyoxylate cycle tricarboxylic acid cycle isocitrate metabolic process 2-oxoglutarate metabolic process NADPH regeneration glutathione metabolic process response to oxidative stress female gonad development response to steroid hormone regulation of phospholipid catabolic process regulation of phospholipid biosynthetic process cell-cell adhesion

Process(es)

magnesium ion binding
isocitrate dehydrogenase (NADP+) activity
receptor binding
protein homodimerization activity
NADP binding
NAD binding
(R)-2-hydroxyglutarate dehydrogenase activity
cadherin binding involved in cell-cell adhesion

Suggested Secondary Antibodies

Anti-Mouse Secondary Antibodies (1824)

Alexa Fluor® Anti-Mouse Secondary Antibodies (219)

HRP Anti-Mouse Secondary Antibodies (234)

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Isocitrate Dehydrogenase 1/IDH1 Mouse anti-Human, Clone: 843219, R&D Systems™

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Isocitrate Dehydrogenase 1/IDH1 Mouse anti-Human, Clone: 843219, R&D Systems™

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Product Details

Product Specific Information

Bioinformatics

Suggested Secondary Antibodies

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Material safety data sheets (MSDS)

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