Invitrogen
TESTED APPLICATIONS | DILUTION |
---|---|
Neutralization (Neu) | Assay-dependent |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Recombinant human interferon alpha A, alpha B2, and alpha F |
Conjugate | Unconjugated |
Form | Liquid |
Storage conditions | -20°C |
RRID | AB_223449 |
Specificity: Neutralizes human interferon alpha.
Assay Used to Measure Bioactivity: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-alpha) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) “Convenient Assay for Interferons,” J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) “A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon,” in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/mL of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-alpha provided by the National Institutes of Health [see Pestka, S. (1986) “Interferon Standards and General Abbreviations,” in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York, 119, 14-23]. This material is prepared specifically for effective neutralization of Hu-IFN-alpha."
Store at -20°C for short-term use (less than 6 months). For long-term storage, aliquot and store at -70°C or below for retention of full activity. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% BSA.
Packaged by and distributed for PBL Assay Science.
IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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