Invitrogen
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA3-012
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Flow Cytometry (Flow) | 1-20 µg/mL |
Immunohistochemistry (Frozen) (IHC (F)) | 10 µg/mL |
Immunohistochemistry (Paraffin) (IHC (P)) | 1:20-1:100 |
Immunoprecipitation (IP) | 2 µg |
Western Blot (WB) | 1:1,000-1:20,000 |
Immunocytochemistry (ICC/IF) | 10-20 µg/mL |
PUBLISHED APPLICATIONS | |
---|---|
Flow Cytometry (Flow) | See 1 publications below |
Western Blot (WB) | See 18 publications below |
Immunoprecipitation (IP) | See 2 publications below |
Immunohistochemistry (IHC) | See 3 publications below |
Immunocytochemistry (ICC/IF) | See 3 publications below |
Species reactivity | Human, Mouse, Non-human primate, Rat |
Published species | Human, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptide corresponding to residues P(2) E E V H H G E E E V E(13) of mouse HSP84. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2121220 |
PA3-012 detects heat shock protein 90 beta (HSP90) from human, monkey, mouse, and rat tissues and cells. This antibody does not detect HSP86.
PA3-012 has been successfully used in Western blot, immunohistochemistry, immunofluorescence, flow cytometry, and immunoprecipitation procedures. By Western blot, this antibody detects an ~84 kDa protein representing HSP84 from Hepa1 cell lysate. Immunofluorescence staining of HSP84 in Hepa1 cells with PA3-012 yields a pattern consistent with cytoplasmic staining. Immunoprecipitation experiments with this antibody suggest that HSP84 exists primarily as homodimers in Hepa 1 cells. Furthermore, the antibody is capable of precipitating HSP84 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor.
The PA3-012 immunogen is a synthetic peptide corresponding to residues P(2) E E V H H G E E E V E(13) of mouse HSP84. The N-terminal regions of HSP84 and HSP86 show the largest difference in amino acid sequence.
Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, aryl hydrocarbon (Ah) receptor, and some kinases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Heat shock 84 kDa; heat shock 90kD protein 1, beta; heat shock 90kDa protein 1, beta; Heat shock protein; heat shock protein 1, beta; heat shock protein 90 kDa; heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein 90kDa alpha family class B member 1; Heat shock protein HSP 90-beta; heat shock protein, 84 kDa 1; HSP; HSP 84; HSP 90; HSP84; HSP90-beta; retinal degeneration slow protein; RP1-302G2.1; TSTA; Tumor-specific transplantation 84 kDa antigen
Gene Aliases: 90kDa; AL022974; C81438; D6S182; HSP84; Hsp84-1; Hsp90; HSP90-BETA; HSP90AB1; HSP90B; HSPC2; HSPCB
UniProt ID: (Human) P08238, (Mouse) P11499, (Rat) P34058
Entrez Gene ID: (Human) 3326, (Mouse) 15516, (Rat) 301252
Molecular Function:
Hsp90 family chaperone
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.