Catalog # MA1-10890
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Flow Cytometry (Flow) | 10 µg/mL |
Immunohistochemistry (IHC) | 1-10 µg/mL |
Immunoprecipitation (IP) | 1-10 µg/mL |
Western Blot (WB) | 1 µg/mL |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 1 publications below |
Species reactivity | Human, Many, Mouse, Rat |
Published species | Mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Clone | N27F3-4 |
Immunogen | Purified recombinant Hsp70/Hsc70. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1.0 mg/mL |
Purification | Protein G |
Storage buffer | PBS, pH 7.4, with 50% glycerol |
Contains | 0.1% sodium azide |
Storage conditions | 4° C |
RRID | AB_906423 |
MA1-10890 detects Heat Shock Protein 70/Heat Shock Cognate Protein 70 in human, mouse, rat, rabbit, monkey, guinea pig, hamster, chicken, bovine, canine, ovine, porcine, fish, Xenopus, Drosophila, C. elegans, and plant samples.
MA1-10890 has been successfully used in Western blot, immunohistochemistry, immunoprecipitation and flow cytometry procedures. By Western blot, MA1-10890 is specific for the ~72 kDa Heat Shock Protein 70/Heat Shock Cognate Protein 70 protein. Positive control of HeLa cell lysate recommended.
MA1-10890 immunogen is purified recombinant Hsp70/Hsc70.
The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. HSC70 (also known as HSC71, HSC73, HSP73, p72, prp73) is expressed constitutively and is slightly heat-inducible. HSC70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. HSC70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AAG2; constitutive heat shock protein 70; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; FAM10A1; Heat shock 70 kDa protein 8; heat shock 70kd protein 10; heat shock 70kD protein 8; heat shock 70kDa protein 8; Heat shock cognate 71 kDa protein; heat shock cognate hsc73; heat shock cognate protein 54; Heat shock cognate protein 70; heat shock protein 8; heat shock protein A8; heat shock protein cognate 70; LAP-1; Lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; N-myristoyltransferase inhibitor protein 71; SNC6
Gene Aliases: 2410008N15Rik; HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; Hsc73; HSP71; HSP73; HSPA10; HSPA8; LAP-1; LAP1; NIP71
UniProt ID: (Human) P11142, (Rat) P63018, (Mouse) P63017
Entrez Gene ID: (Human) 3312, (Rat) 24468, (Mouse) 15481
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