Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA1-860
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
ChIP assay (ChIP) | 2.5 µg/10^6 cells |
Immunohistochemistry (Frozen) (IHC (F)) | 0.2 µg/mL |
Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1 µg/mL |
Immunocytochemistry (ICC/IF) | 2.0 µg/mL |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 36 publications below |
in situ PLA (PLA) | See 1 publications below |
Immunohistochemistry (IHC) | See 9 publications below |
ChIP assay (ChIP) | See 12 publications below |
Immunohistochemistry (Paraffin) (IHC (P)) | See 2 publications below |
Immunoprecipitation (IP) | See 8 publications below |
Immunocytochemistry (ICC/IF) | See 7 publications below |
Immunohistochemistry - Free Floating (IHC (Free)) | See 1 publications below |
Miscellaneous PubMed (Misc) | See 1 publications below |
Species reactivity | Dog, Hamster, Human, Mouse, Rat |
Published species | Chimpanzee, Fruit fly, Human, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptide corresponding to residues C E(467) E K P E A K G V K E E V K L A(482) of human HDAC1. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2118091 |
PA1-860 detects histone deacetylase 1 (HDAC1) from human, mouse, rat, hamster and canine tissues and cells.
PA1-860 has been successfully used in Western blot, immunoprecipitation, ChIP, immunofluoresence, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~62 kDa protein representing HDAC1 from HeLa cell lysate. Immunocytochemical staining of HDAC1 in HeLa cells with PA1-860 results in nuclear staining.
The PA1-860 immunogen is a synthetic peptide corresponding to residues C E(467) E K P E A K G V K E E V K L A(482) of human HDAC1. This immunizing peptide (Cat. # PEP-069) is available for use in neutralization and control experiments.
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DKFZp686H12203; HD1; Histone deacetylase 1; histone deacetylase 1-like protein; Protein deacetylase HDAC1; Protein decrotonylase HDAC1; reduced potassium dependency, yeast homolog-like 1; RP4-811H24.2
Gene Aliases: GON-10; HD1; HDAC1; Hdac1-ps; MommeD5; RPD3; RPD3L1
UniProt ID: (Human) Q13547, (Mouse) O09106, (Rat) Q4QQW4
Entrez Gene ID: (Human) 3065, (Dog) 487309, (Mouse) 433759, (Rat) 297893, (Chinese hamster) 100752721
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