Catalog # MA1-46412
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Flow Cytometry (Flow) | 1 µg per million cells |
Immunohistochemistry (Frozen) (IHC (F)) | 0.5-1.0 µg/mL |
Immunohistochemistry (Paraffin) (IHC (P)) | 0.5-1.0 µg/mL |
Immunoprecipitation (IP) | 1:10-1:500 |
Western Blot (WB) | 0.5-1.0 µg/mL |
Immunocytochemistry (ICC/IF) | Assay-Dependent |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 3 publications below |
Immunohistochemistry (Paraffin) (IHC (P)) | See 1 publications below |
Species reactivity | Human, Mouse, Rat |
Published species | Human, Rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Clone | 1B6 |
Immunogen | Fusion protein from rat HAP1. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Protein G |
Storage buffer | PBS with 50% glycerol |
Contains | 0.05% sodium azide |
Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID | AB_2232841 |
In Western blot bands can be seen ~95 kDa for isoform A and ~110 kDa for isoform B.
Suggested positive control: rat brain.
Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. HAP1 was initially identified through a two-hybrid library screening; the binding of HAP1 to huntingtin correlated with the expansion of the polyglutamine tract. HAP1 also interacts with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), suggesting that HAP1 may play a role in vesicular trafficking or organelle transport. HAP1 is also involved with the huntingtin-enhanced BDNF transport along the cellular microtubles. Attenuation of this process led to the loss of neurotrophic support and neuronal toxicity, which suggests that loss of this function might contribute to pathogenesis. Several alternatively spliced isoforms have been described for HAP1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: HAP-1; HAP-1 antibody; Huntingtin-associated protein 1; huntingtin-associated protein 2; Neuroan 1
Gene Aliases: HAP-1; HAP1; HAP1-A; HAP1-B; HAP2; hHLP1; HIP5; HLP; HLP1
UniProt ID: (Human) P54257, (Rat) P54256, (Mouse) O35668
Entrez Gene ID: (Human) 9001, (Rat) 29430, (Mouse) 15114
Molecular Function:
membrane traffic protein
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