Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA5-40134
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Paraffin) (IHC (P)) | 1:100-1:1,000 |
Immunoprecipitation (IP) | 1:100-1:500 |
Western Blot (WB) | 1:1,000-1:10,000 |
Immunocytochemistry (ICC/IF) | 1:100-1:1,000 |
Species reactivity | Human, Mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Glutaminase C. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 0.5 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS, pH 7, with 20% glycerol |
Contains | 0.025% ProClin 300 |
Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID | AB_2609956 |
This antibody specifically recognizes GAC but not the KGA isoform.
Predicted reactivity: Bovine (100%).
Recommended positive controls: MDA-MB-231 and cholangiocarcinoma tissue.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GLS; glutaminase C; Glutaminase kidney isoform, mitochondrial; glutaminase, phosphate-activated; K-glutaminase; L-glutamine amidohydrolase
Gene Aliases: 6330442B14; AAD20; AI314027; B230365M23Rik; GAC; GAM; GLS; GLS1; KGA; KIAA0838
UniProt ID: (Human) O94925, (Mouse) D3Z7P3
Entrez Gene ID: (Human) 2744, (Mouse) 14660
Molecular Function:
hydrolase
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