Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA1-516
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
ChIP assay (ChIP) | Assay-dependent |
Immunohistochemistry (Paraffin) (IHC (P)) | 1:20 |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 4 µg/mL |
Immunocytochemistry (ICC/IF) | Assay-dependent |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 17 publications below |
ChIP assay (ChIP) | See 7 publications below |
Immunocytochemistry (ICC/IF) | See 8 publications below |
Flow Cytometry (Flow) | See 2 publications below |
Immunohistochemistry (IHC) | See 3 publications below |
Immunoprecipitation (IP) | See 1 publications below |
Gel Shift (GS) | See 1 publications below |
Species reactivity | Human, Mouse, Rat |
Published species | Human, Mouse, Non-human primate, Plant, Rat, Sheep |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic Peptide: C(755) E I I T N Q I P K Y S N G N I K K(771) |
Conjugate | Unconjugated |
Form | Lyophilized |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2251653 |
PA1-516 detects glucocorticoid receptor (GR) alpha from human, mouse and rat tissues. This antibody does not detect GR beta.
PA1-516 has been successfully used in Western blot, immunocytochemistry, immunofluorescence and immunoprecipitation (ChIP) procedures. By Western blot, this antibody detects an ~94 kDa protein representing GR in HeLa cell lysate.
PA1-516 immunizing peptide corresponds to amino acid residues 755-771 of human GR alpha. This peptide (Cat. # PEP-221) is available for use in neutralization and control experiments.
Reconstitute with 50 µL of distilled water.
Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; glucocorticoid receptor 1; GR; gr alpha; GR Kit; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858
Gene Aliases: GCCR; GCR; GCRST; GR; GRL; Grl-1; Grl1; NR3C1
UniProt ID: (Human) P04150, (Mouse) P06537, (Rat) P06536
Entrez Gene ID: (Human) 2908, (Mouse) 14815, (Rat) 24413
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