Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # MA1-510
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Flow Cytometry (Flow) | 1-2 µg/test |
Gel Shift (GS) | Assay-dependent |
Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:500 |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 5 µg/mL |
Immunocytochemistry (ICC/IF) | Assay-dependent |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 72 publications below |
Immunohistochemistry (IHC) | See 9 publications below |
ChIP assay (ChIP) | See 19 publications below |
Flow Cytometry (Flow) | See 4 publications below |
Immunoprecipitation (IP) | See 22 publications below |
Immunohistochemistry (Frozen) (IHC (F)) | See 1 publications below |
Miscellaneous PubMed (Misc) | See 1 publications below |
Immunocytochemistry (ICC/IF) | See 13 publications below |
Gel Shift (GS) | See 5 publications below |
ELISA (ELISA) | See 2 publications below |
Species reactivity | Guinea pig, Human, Mouse, Sheep, Rabbit, Rat, Yeast |
Published species | Human, Mouse, Non-human primate, Rabbit, Rat, Sheep, Xenopus, Yeast |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Clone | BuGR2 |
Immunogen | Partially purified rat GR. |
Conjugate | Unconjugated |
Form | Lyophilized |
Concentration | 1 mg/mL |
Purification | Protein A |
Storage buffer | PBS, pH 7.2 |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_325427 |
MA1-510 detects glucocorticoid receptor (GR) from human, mouse, rat, guinea pig, rabbit, sheep and yeast samples. This antibody does not react with primate, avian or amphibian GR.
MA1-510 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, flow cytometry, immunohistochemistry, immunoprecipitation, and gel shift procedures. By Western blot, this antibody detects a 97 kDa protein representing GR in L929 cell extract. Immunocytochemical staining of GR in L929 cells with MA1-510 results in staining of both the cytoplasm and nucleus, even in the presence of hormone. Using enzymatic digestion analysis, MA1-510 reacts with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.
The MA1-510 immunogen is partially purified rat GR.
Reconstitute with 100 µL distilled water.
Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cytosolic/nuclear receptor; glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; glucocorticoid receptor 1; glucocorticoid receptor alpha; GR; gr alpha; GR Kit; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858
Gene Aliases: GCCR; GCR; GCRST; GR; GRL; Grl-1; Grl1; NR3C1
UniProt ID: (Rabbit) P59667, (Human) P04150, (Mouse) P06537, (Guinea pig) P49115, (Rat) P06536
Entrez Gene ID: (Sheep) 443412, (Rabbit) 100008890, (Human) 2908, (Mouse) 14815, (Guinea pig) 100135583, (Rat) 24413
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