Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA1-511A
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
Western Blot (WB) | 5 µg/mL |
Immunocytochemistry (ICC/IF) | 1 µg/mL |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 39 publications below |
ChIP assay (ChIP) | See 25 publications below |
Immunohistochemistry (IHC) | See 26 publications below |
Immunohistochemistry (Frozen) (IHC (F)) | See 2 publications below |
Immunocytochemistry (ICC/IF) | See 9 publications below |
Flow Cytometry (Flow) | See 1 publications below |
ChIP-sequencing (ChIP-Seq) | See 5 publications below |
Immunoprecipitation (IP) | See 3 publications below |
Immunohistochemistry (Paraffin) (IHC (P)) | See 1 publications below |
Neutralization (Neu) | See 1 publications below |
Gel Shift (GS) | See 1 publications below |
Species reactivity | Human, Mouse, Rat |
Published species | Bovine, Chicken, Human, Mouse, Non-human primate, Rabbit, Rat, Sheep, Yeast |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptide corresponding to residues D(346) Q K P I F N V I P P I P V G S E N W N R C(367) from human GR. |
Conjugate | Unconjugated |
Form | Lyophilized |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS |
Contains | no preservative |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2236340 |
PA1-511A detects glucocorticoid receptor (GR) from human, mouse and rat tissues.
For reconstitution, centrifuge the vial briefly, then add 50 µL PBS to achieve a final antibody concentration of 1 mg/mL.
PA1-511A has been successfully used in Western blot, immunocytochemistry, immunofluorescence, and immunohistochemistry procedures. By Western blot, this antibody detects an ~97 kDa protein representing GR from rat liver extract. Immunocytochemical staining of GR in HeLa cells using PA1-511A results in cytoplasmic staining in the absence of ligand, and nuclear staining after hormone administration.
The PA1-511A immunogen is a synthetic peptide corresponding to residues D(346) Q K P I F N V I P P I P V G S E N W N R C(367) from human GR. The PA1-511A immunizing peptide (Cat. # PEP-001) is available for use in neutralization and control experiments.
Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; glucocorticoid receptor 1; GR; gr alpha; GR Kit; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858
Gene Aliases: GCCR; GCR; GCRST; GR; GRL; Grl-1; Grl1; NR3C1
UniProt ID: (Human) P04150, (Mouse) P06537, (Rat) P06536
Entrez Gene ID: (Human) 2908, (Mouse) 14815, (Rat) 24413
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