Invitrogen
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. View details.
Catalog # MA1-720
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (IHC) | Assay-dependent |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1 µg/mL |
Immunocytochemistry (ICC/IF) | 2-3 µg/mL |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 16 publications below |
Immunohistochemistry (IHC) | See 4 publications below |
Immunocytochemistry (ICC/IF) | See 2 publications below |
Species reactivity | Bovine, Chicken, Human, Mouse, Rat |
Published species | Bovine, Dog, Human, Mouse, Rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Clone | G8 |
Immunogen | Full length human GRK1. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Protein G |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2115613 |
MA1-720 detects G-protein-associated rhodopsin kinase 1a (GRK1a) from human, mouse, rat, bovine and chicken tissues.
MA1-720 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~60 kDa protein representing GRK1a from bovine rod outer segments. Immunofluorescence staining of GRK1a in bovine retina with MA1-720 results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods.
The MA1-720 immunogen is full length human GRK1. This antibody recognizes an epitope in the C-terminal region of GRK1a.
GRK1 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinases. GRK1 mediates rapid desensitization of rod photoreceptors to light by catalyzing phosphorylation of the visual pigment rhodopsin which leads to its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). GRK1 can be phosphorylated by PKA and this reduces the ability of GRK1 to phosphorylate rhodopsin in vitro. It is speculated that phosphorylation of GRK1 by PKA in vivo occurs in the dark when cAMP levels in photoreceptor cells are elevated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: G protein-coupled receptor kinase 1; G protein-coupled receptpr kinase 1; G-protein receptor kinase 1; GRK 1a/b; GRK1a; rhodopsin kinase; Rhodopsin kinase GRK1; RK
Gene Aliases: GPRK1; GRK1; RHOK; RK
UniProt ID: (Human) Q15835, (Bovine) P28327, (Rat) Q63651
Entrez Gene ID: (Human) 6011, (Chicken) 395431, (Bovine) 281457, (Mouse) 24013, (Rat) 81760
Molecular Function: non-receptor serine/threonine protein kinase
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.