Catalog # PA3-843
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1:2,000 |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 2 publications below |
Immunoprecipitation (IP) | See 1 publications below |
Species reactivity | Human |
Published species | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Full-length human E6-AP:GST fusion protein. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | Conc. Not Determined |
Storage buffer | whole serum, PBS |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2211788 |
PA3-843 detects Angelman Syndrome-Associated protein (E6-AP) from human tissues.
PA3-843 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a single ~100 kDa protein representing E6-AP from HeLa cell lysate.
IMPORTANT: E6-AP is a very labile protein. Freeze/thaw cycling of samples results in E6-AP degradation which is observed as a prominent 50 kDa band seen in Western blots.
PA3-843 antigen is bacterially expressed, full-length human E6-AP.
UBE3A (ubiquitin-protein ligase E3A, AKA E6AP) is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the ARNTL/BMAL1, ARC, RAD23A, RAD23B, MCM7, annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, UBE3A may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. UBE3A also plays an important role in the regulation of the circadian clock. Defects in UBE3A are a cause of Angelman syndrome (AS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CTCL tumor antigen se37-2; E6AP ubiquitin-protein ligase; FLJ26981; HECT-type ubiquitin transferase E3A; human papilloma virus E6-associated protein; Human papillomavirus E6-associated protein; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54; Ubiquitin-protein ligase E3A
Gene Aliases: ANCR; AS; E6-AP; E6AP; EPVE6AP; HPVE6A; UBE3A
UniProt ID: (Human) Q05086
Entrez Gene ID: (Human) 7337
Molecular Function:
ubiquitin-protein ligase
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