Catalog # MA1-070
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Flow Cytometry (Flow) | 1-2 µg/test |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1 µg/mL |
RNA Immunoprecipitation (RIP) | Assay-dependent |
Immunocytochemistry (ICC/IF) | 2-4 µg/mL |
PUBLISHED APPLICATIONS | |
---|---|
Flow Cytometry (Flow) | See 1 publications below |
Western Blot (WB) | See 4 publications below |
Immunocytochemistry (ICC/IF) | See 1 publications below |
Immunoprecipitation (IP) | See 2 publications below |
Species reactivity | Bovine, Human, Many, Mouse, Rat |
Published species | Human, Mouse, Rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Clone | 74.1 |
Immunogen | Purified bovine brain cytoplasmic dynein. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Protein A |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2093668 |
MA1-070 detects two isoforms of the ~74 kDa polypetide of cytoplasmic dynein.
MA1-070 has been successfully used in Western blot immunofluorescence, and immunoprecipitation procedures. In Western blot analysis of HeLa cell lysate this antibody detects a ~74 kDa protein representing cytoplasmic dynein.
The MA1-070 immunogen is purified bovine brain cytoplasmic dynein.
The directed movement of membrane-bounded organelles is an important process for various cellular functions including membrane transport, secretion, and axonal transport. Cytoplasmic dynein, a ubiquitous minus end-directed microtubule-based motor protein, is believed to be responsible for the retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport. The cytoplasmic dynein complex is composed of two ~530 kDa heavy chains, and intermediate chains of ~74 kDa and ~53-59 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 8 kDa dynein light chain; 8 kDa dynein light chain b; 8kD LC; 8kDa LC; cytoplasmic dynein light polypeptide; cytosolic DH IC-1; DLC8; DLC8b; DNCL1; dynein LC8; Dynein light chain 1, cytoplasmic; Dynein light chain 2, cytoplasmic; Dynein light chain LC8-type 1; Dynein light chain LC8-type 2; dynein light chain-2; dynein, cytoplasmic, light chain 1; dynein, cytoplasmic, light peptide; dynein, cytoplasmic, light polypeptide 1; dynein, light chain, LC8-type 1; dynein, light chain, LC8-type 2; mPIN; PIN; Protein inhibitor of neuronal nitric oxide synthase; protein inhibitor of nitric oxide synthase; radial spoke 22 homolog
Gene Aliases: 1700064A15Rik; 6720463E02Rik; 8kDLC; C87222; CDLC2; DLC1; DLC2; DLC8; DLC8b; DNCL1; DNCL1B; DNCLC1; DYNLL1; DYNLL2; HDLC1; LC8; LC8a; PIN; RSPH22
UniProt ID: (Human) P63167, (Bovine) P61285, (Human) Q96FJ2, (Bovine) Q3MHR3, (Rat) P63170, (Mouse) P63168, (Rat) Q78P75, (Mouse) Q9D0M5
Entrez Gene ID: (Human) 8655, (Bovine) 404151, (Human) 140735, (Bovine) 540369, (Rat) 58945, (Mouse) 56455, (Rat) 140734, (Mouse) 68097
Molecular Function:
microtubule or microtubule-binding cytoskeletal protein
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