R&D Systems

Manufacturer Code:MAB7934SP

Dihydrofolate Reductase/DHFR Mouse anti-Human, Mouse, Rat, Clone: 872442, R&D Systems™

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Dihydrofolate Reductase/DHFR in C2C12 Mouse Cell Line. Dihydrofolate Reductase/DHFR was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Dihydrofolate Reductase/DHFR Monoclonal Antibody (Catalog # MAB7934) at 10 g/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Dihydrofolate Reductase/DHFR in MCF-7 Human Cell Line. Dihydrofolate Reductase/DHFR was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Dihydrofolate Reductase/DHFR Monoclonal Antibody (Catalog # MAB7934) at 10 g/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Dihydrofolate Reductase/DHFR in Human Colon. Dihydrofolate Reductase/DHFR was detected in formalin fixed paraffin-embedded sections of human colon tissue using Mouse Anti-Human Dihydrofolate Reductase/DHFR Monoclonal Antibody (Catalog # MAB7934) at 15 g/mL overnight at 4 C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human, Mouse, and Rat Dihydrofolate Reductase/DHFR by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 g/mL of Mouse Anti-Human Dihydrofolate Reductase/DHFR Monoclonal Antibody (Catalog # MAB7934) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Dihydrofolate Reductase/DHFR at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Dihydrofolate Reductase/DHFR by Simple Western<sup>TM</sup>. — Detection of Human Dihydrofolate Reductase/DHFR by Simple Western^TM. Simple Western lane view shows lysates of 293T human embryonic kidney cell line, loaded at 0.5 mg/mL. A specific band was detected for Dihydrofolate Reductase/DHFR at approximately 28 kDa (as indicated) using 10 g/mL of Mouse Anti-Human/Mouse/Rat Dihydrofolate Reductase/DHFR Monoclonal Antibody (Catalog # MAB7934). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Product Details

TESTED APPLICATIONS	DILUTION
Immunocytochemistry (ICC)	Assay dependent
Immunohistochemistry (IHC)	Assay dependent
Western Blot (WB)	Assay dependent

Species reactivity	Many
Host / Isotype	Mouse
Class	Monoclonal
Type	Antibody
Clone	872442
Immunogen	<EM>E. coli</EM>-derived recombinant human Dihydrofolate Reductase/DHFR Met1-Asp187 Accession # P00374
Conjugate	Unconjugated
Form	Liquid
Purification	purified
Storage buffer	PBS, pH7.4
Contains	15mM sodium azide
Storage conditions	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt -20 to -70 degreesC as supplied. 1 month 2 to 8 degreesC under sterile conditions after reconstitution. 6 months -20 to -70 degreesC under sterile con

Product Specific Information

Mouse Monoclonal Antibody

For Research Use Only

Bioinformatics

Protein Aliases: DHFR DHFRP1 dihydrofolate reductase DYR EC 1.5.1.3; Dihydrofolate reductase

Gene Aliases: DHFR; DHFRP1; DYR

UniProt ID: (Human) P00374

Entrez Gene ID: (Human) 1719

Molecular Function: Blue Arrow Icon oxidoreductase reductase

Function(s)

G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle glycine biosynthetic process tetrahydrobiopterin biosynthetic process one-carbon metabolic process nucleotide biosynthetic process axon regeneration response to methotrexate dihydrofolate metabolic process tetrahydrofolate metabolic process tetrahydrofolate biosynthetic process folic acid metabolic process positive regulation of nitric-oxide synthase activity oxidation-reduction process regulation of removal of superoxide radicals

Process(es)

mRNA binding
dihydrofolate reductase activity
folic acid binding
drug binding
folate reductase activity
methotrexate binding
NADPH binding

Suggested Secondary Antibodies

Anti-Mouse Secondary Antibodies (1824)

Alexa Fluor® Anti-Mouse Secondary Antibodies (219)

HRP Anti-Mouse Secondary Antibodies (234)

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Dihydrofolate Reductase/DHFR Mouse anti-Human, Mouse, Rat, Clone: 872442, R&D Systems™

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Dihydrofolate Reductase/DHFR Mouse anti-Human, Mouse, Rat, Clone: 872442, R&D Systems™

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Product Details

Product Specific Information

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

Performance Guarantee

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Your items have has been added!