R&D Systems

Manufacturer Code:AF7934SP

Dihydrofolate Reductase/DHFR Sheep anti-Human, Polyclonal, R&D Systems™

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Catalog # AF7934SP

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Dihydrofolate Reductase/DHFR in MCF-7 Human Cell Line. Dihydrofolate Reductase/ DHFR was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human Dihydrofolate Reductase/ DHFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7934) at 10 g/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Dihydrofolate Reductase/DHFR by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, HEK293T human embryonic kidney cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 g/mL of Sheep Anti-Human Dihydrofolate Reductase/DHFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7934) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dihydrofolate Reductase/DHFR at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Product Details

TESTED APPLICATIONS	DILUTION
Immunocytochemistry (ICC)	Assay dependent
Western Blot (WB)	Assay dependent

Species reactivity	Human
Host / Isotype	Sheep
Class	Polyclonal
Type	Antibody
Immunogen	<EM>E. coli</EM>-derived recombinant human Dihydrofolate Reductase/DHFR Met1-Asp187 Accession # P00374
Conjugate	Unconjugated
Form	Liquid
Purification	purified
Storage buffer	PBS, pH7.4
Contains	15mM sodium azide
Storage conditions	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt -20 to -70 degreesC as supplied. 1 month 2 to 8 degreesC under sterile conditions after reconstitution. 6 months -20 to -70 degreesC under sterile con

Product Specific Information

Sheep Polyclonal Antibody

For Research Use Only

Bioinformatics

Protein Aliases: DHFR DHFRP1 dihydrofolate reductase DYR EC 1.5.1.3; Dihydrofolate reductase

Gene Aliases: DHFR; DHFRP1; DYR

UniProt ID: (Human) P00374

Entrez Gene ID: (Human) 1719

Molecular Function: Blue Arrow Icon oxidoreductase reductase

Function(s)

G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle glycine biosynthetic process tetrahydrobiopterin biosynthetic process one-carbon metabolic process nucleotide biosynthetic process axon regeneration response to methotrexate dihydrofolate metabolic process tetrahydrofolate metabolic process tetrahydrofolate biosynthetic process folic acid metabolic process positive regulation of nitric-oxide synthase activity oxidation-reduction process regulation of removal of superoxide radicals

Process(es)

mRNA binding
dihydrofolate reductase activity
folic acid binding
drug binding
folate reductase activity
methotrexate binding
NADPH binding

Suggested Secondary Antibodies

Anti-Sheep Secondary Antibodies (177)

Alexa Fluor® Anti-Sheep Secondary Antibodies (1)

HRP Anti-Sheep Secondary Antibodies (31)

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Dihydrofolate Reductase/DHFR Sheep anti-Human, Polyclonal, R&D Systems™

Product Details

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Bioinformatics

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Dihydrofolate Reductase/DHFR Sheep anti-Human, Polyclonal, R&D Systems™

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Product Details

Product Specific Information

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

Performance Guarantee

We’re here to help

Your items have has been added!