Invitrogen
Catalog # PA1-84583
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
ChIP assay (ChIP) | 2.5 µg/10^6 cells |
Gel Shift (GS) | Assay-dependent |
Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1:2,000 |
Immunocytochemistry (ICC/IF) | 1:250 |
Species reactivity | Hamster, Human, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Human DNA-PKcs fragment. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | Conc. Not Determined |
Storage buffer | whole serum |
Contains | <0.1% sodium azide |
Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID | AB_2172840 |
Heat-mediated antigen retrieval using an antigen unmasking fluid is recommended, followed by a 2-hr incubation with primary antibody.
Rabbit anti DNA PKcs antibody recognizes the human DNA-dependent protein kinase catalytic subunit, also known as DNA-PKcs, DNPK1 or p460.
DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. DNA-PK is an atypical kinase, closely related to the PI3 kinases, and is often potently inhibited by small molecules designed to target PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for DNA-PK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNPK1; doxorubicin nephropathy; hyper-radiosensitivity of murine scid mutation, complementing 1; p460; protein kinase, DNA activated, catalytic polypeptide; protein kinase, DNA-activated, catalytic polypeptide; severe combined immunodeficiency
Gene Aliases: AI326420; AU019811; DNA-PKcs; DNAPDcs; DNAPK; DNPK1; DOXNPH; dxnph; HYRC; HYRC1; I79_017613; IMD26; p350; p460; PRKDC; scid; slip; XRCC7
UniProt ID: (Human) P78527, (Mouse) P97313
Entrez Gene ID: (Human) 5591, (Chinese hamster) 100770748, (Mouse) 19090, (Rat) 360748
Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme
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