Proteintech

Manufacturer Code:151941AP150UL

DHFR Rabbit anti-Human, Mouse, Rat, Polyclonal, Proteintech

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Catalog # 50-173-0055

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DHFR Antibody (151941AP) in IF — Immunofluorescent analysis of HeLa cells using 15194-1-AP (DHFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

DHFR Antibody (151941AP) in IHC (P) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15194-1-AP ( DHFR Antibody) at dilution of 1:50 (under 10x lens).

DHFR Antibody (151941AP) in WB — Jurkat cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DHFR Antibody (151941AP) in IP — IP result of anti-DHFR (IP:15194-1-AP, 3ug; Detection:15194-1-AP 1:500) with Raji cells lysate 2000ug.

Product Details

TESTED APPLICATIONS	DILUTION
Immunocytochemistry (ICC)	Assay dependent
Immunofluorescence (IF)	Assay dependent
Immunohistochemistry (Paraffin) (IHC (P))	Assay dependent
Immunoprecipitation (IP)	Assay dependent
Western Blot (WB)	Assay dependent

Species reactivity	Many
Host / Isotype	Rabbit
Class	Polyclonal
Type	Antibody
Immunogen	DHFR Fusion Protein Ag7331
Conjugate	Unconjugated
Form	Liquid
Purification	purified
Storage buffer	PBS, pH7.4
Contains	15mM sodium azide
Storage conditions	-20°C

Product Specific Information

Rabbit Polyclonal Antibody

For Research Use Only

Bioinformatics

Protein Aliases: DHFR DHFRP1 dihydrofolate reductase; Dihydrofolate reductase; Dihydrofolate reductase 1 (active)

Gene Aliases: 8430436I03Rik; AA607882; AI662710; AW555094; DHFR; Dhfr1; DHFRP1; DYR

UniProt ID: (Human) P00374, (Mouse) P00375, (Rat) Q920D2

Entrez Gene ID: (Human) 1719, (Mouse) 13361, (Rat) 24312

Molecular Function: Blue Arrow Icon oxidoreductase reductase

Function(s)

G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle glycine biosynthetic process tetrahydrobiopterin biosynthetic process one-carbon metabolic process nucleotide biosynthetic process axon regeneration response to methotrexate dihydrofolate metabolic process tetrahydrofolate metabolic process tetrahydrofolate biosynthetic process folic acid metabolic process positive regulation of nitric-oxide synthase activity oxidation-reduction process regulation of removal of superoxide radicals response to nicotine

Process(es)

mRNA binding
dihydrofolate reductase activity
folic acid binding
drug binding
folate reductase activity
methotrexate binding
NADPH binding
RNA binding
oxidoreductase activity
NADP binding
dihydrofolic acid binding

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DHFR Rabbit anti-Human, Mouse, Rat, Polyclonal, Proteintech

Product Details

Product Specific Information

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

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DHFR Rabbit anti-Human, Mouse, Rat, Polyclonal, Proteintech

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Product Details

Product Specific Information

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

Performance Guarantee

We’re here to help

Your items have has been added!