Invitrogen
Catalog # PA1-4650
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (IHC) | 1:250 |
Western Blot (WB) | 1:1,000 |
Immunocytochemistry (ICC/IF) | 1:250 |
Species reactivity | Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptide corresponding to the N-terminal region of rat PPP1R1B conjugated to KLH |
Conjugate | Unconjugated |
Form | Liquid |
Purification | Affinity chromatography |
Storage buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
Contains | no preservative |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_558596 |
This antibody is predicted to react with bovine, canine, chicken, human, mouse, non-human primate and Xenopus based on 100% sequence homology.
This antibody is specific for the ~32 kDa DARPP-32 protein.
DARPP-32 is a dopamine (DA) and AMP-regugated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons. The protein inhibits Protein Phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regugation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein; dopamine- and cAMP-regulated phosphoprotein DARPP-32; FLJ20940; IPPD; neuronal phosphoprotein DARPP-32; OTTHUMP00000164275; OTTHUMP00000164276; PPR1B; protein pho; Protein phosphatase 1 regulatory subunit 1B
Gene Aliases: AU040756; DARPP-32; Darpp32; Ppp1r1b
UniProt ID: (Rat) Q6J4I0, (Mouse) Q60829
Entrez Gene ID: (Rat) 360616, (Mouse) 19049
Molecular Function:
phosphatase inhibitor
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