Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View details.
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Frozen) (IHC (F)) | 1:10-1:40 |
Immunohistochemistry (Paraffin) (IHC (P)) | 1:10-1:40 |
Western Blot (WB) | 1:200-1:1,000 |
Immunocytochemistry (ICC/IF) | 1:100 |
PUBLISHED APPLICATIONS | |
---|---|
Immunohistochemistry (IHC) | See 74 publications below |
Immunohistochemistry (Paraffin) (IHC (P)) | See 15 publications below |
in situ PLA (PLA) | See 1 publications below |
Immunocytochemistry (ICC/IF) | See 10 publications below |
Flow Cytometry (Flow) | See 1 publications below |
Miscellaneous PubMed (Misc) | See 2 publications below |
Western Blot (WB) | See 2 publications below |
Published species | Chicken, Horse, Human, Mouse, Non-human primate, Rat, Sheep, Zebrafish |
Host / Isotype | Mouse / IgG1 |
Class | Cocktail |
Type | Antibody |
Clone | AE1/AE3 |
Immunogen | Human epidermal keratins. |
Conjugate | Unconjugated |
Form | Liquid |
Storage conditions | 4°C or -20°C if preferred |
RRID | AB_2281092 |
Heat-mediated antigen retrieval is recommended for the staining of paraffin sections. A suggested positive control is human skin.
This antibody detects the acidic and basic (Type I and II) cytokeratins: Cytokeratin 1, Cytokeratin 2, Cytokeratin 3, Cytokeratin 4, Cytokeratin 5, Cytokeratin 6, Cytokeratin 7, Cytokeratin 8, Cytokeratin 10, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16 and Cytokeratin 19.
Mouse anti Human Cytokeratin (Pan) antibody, clone AE1/AE3 is a pan-cytokeratin reagent, being a cocktail of clones AE1 and AE3 which provides the broadest spectrum of reactivity to the 19 known human epidermal keratins.
Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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