Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA3-022
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Flow Cytometry (Flow) | Assay-dependent |
Gel Shift (GS) | Assay-dependent |
Immunohistochemistry (Frozen) (IHC (F)) | 1:100 |
Immunohistochemistry (Paraffin) (IHC (P)) | 1:20-1:200 |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1:1,000 |
Immunocytochemistry (ICC/IF) | Assay-dependent |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 29 publications below |
Immunocytochemistry (ICC/IF) | See 3 publications below |
Gel Shift (GS) | See 1 publications below |
Immunoprecipitation (IP) | See 2 publications below |
Immunohistochemistry (IHC) | See 1 publications below |
Species reactivity | Human, Mouse, Rat |
Published species | Bovine, Chicken, Hamster, Human, Mouse, Non-human primate, Rabbit, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptide corresponding to residues C A(356) Q K D K E K A V Y A K M F A(370) of human Cyp-40. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Affinity chromatography |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2169452 |
PA3-022 detect cyclophilin 40 (CyP-40) from human and rat and mouse tissues and cells. This antibody does not cross-react with CyPA.
PA3-022 has been successfully used in Western blot, iF, immunohistochemistry, immunocytochemistry, immunoprecipitation, gel shift and FACS procedures. By Western blot, this antibody detects a 40 kDa protein representing CyP-40 from rat brain extract.
The PA3-022 immunogen is a synthetic peptide corresponding to residues C A(356) Q K D K E K A V Y A K M F A(370) of human Cyp-40.
This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 40 kDa peptidyl-prolyl cis-trans isomerase; 40 kDa peptidyl-prolyl cis-trans isomerase D; cyclophilin 40; Cyclophilin D; Cyclophilin-40; Cyclophilin-related protein; Cyp 40; Cyp D; CYP-40; cytoplasmic cyclophilin D; EC 5.2.1.8; MGC33096; Peptidyl-prolyl cis-trans isomerase D; PPIase D; rotamase; Rotamase D; testicular tissue protein Li 147
Gene Aliases: 4930564J03Rik; CYP-40; CYP40; CYPD; PPID; Ppidl; Ppif
UniProt ID: (Human) Q08752, (Mouse) Q9CR16, (Rat) Q6DGG0
Entrez Gene ID: (Human) 5481, (Mouse) 67738, (Rat) 361967
Molecular Function:
chaperone
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