Invitrogen

Manufacturer Code:MA516356

Cyclin D1 Rabbit anti-Human, Mouse, Rat, Invitrogen

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Catalog # PIMA516356

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Cyclin D1 Antibody (MA1-39546) in IF — Immunofluorescence analysis of Cyclin D1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cyclin D1 Rabbit Monoclonal Antibody (Product ## MA1-39546) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Sec

Cyclin D1 Rabbit [MA139546] — Immunohistochemical analysis of Cyclin D1 using anti-Cyclin D1 Monoclonal Antibody (MA5-16356) in Mantle Cell Lymphoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.

Cyclin D1 Antibody (MA5-16356) in WB — Knockdown of Cyclin D1 was achieved by transfecting HeLa cells with Cyclin D1 specific validated siRNAs (Silencer® select Product ## s230). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the Cyclin D1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Cyclin D1 Monoclonal Antibody (Product ## MA5-16356, 1:500 dilution) and Goat anti-Rabbit IgG (H+L) Super

Cyclin D1 Antibody (MA5-16356) in KD — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Cyclin D1 siRNA and decrease in signal was observed in Western Blot using Anti-Cyclin D1 Monoclonal Antibody (Product ## MA5-16356).

Product Details

TESTED APPLICATIONS	DILUTION
Immunocytochemistry (ICC)	Assay dependent
Immunofluorescence (IF)	Assay dependent
Immunohistochemistry (Paraffin) (IHC (P))	Assay dependent
Western Blot (WB)	Assay dependent

Species reactivity	Many
Host / Isotype	Rabbit
Class	Monoclonal
Type	Antibody
Clone	SP4
Immunogen	Synthetic peptide from C-terminus of human cyclin D1.
Conjugate	Unconjugated
Form	Liquid
Purification	purified
Storage buffer	PBS, pH7.4
Contains	15mM sodium azide
Storage conditions	-20&deg C Avoid Freeze/Thaw Cycles

Product Specific Information

Rabbit Recombinant Monoclonal Antibody

For Research Use Only

Bioinformatics

Protein Aliases: B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; BCL1 D11S287E PRAD1 U21B31 B-cell CLL/lymphoma 1 B-cell lymphoma 1 protein BCL-1 oncogene G1/S-specific cyclin-D1 PRAD1 oncogene; G1/S-specific cyclin-D1; PRAD1 oncogene

Gene Aliases: AI327039; bcl-1; BCL1; CCND1; cD1; CycD1; Cyl-1; D11S287E; PRAD1; U21B31

UniProt ID: (Human) P24385, (Rat) P39948, (Mouse) P25322

Entrez Gene ID: (Human) 595, (Rat) 58919, (Mouse) 12443

Molecular Function: Blue Arrow Icon enzyme modulator kinase activator kinase modulator

Function(s)

G1/S transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter re-entry into mitotic cell cycle positive regulation of protein phosphorylation transcription, DNA-templated protein phosphorylation cellular response to DNA damage stimulus lactation response to iron ion response to X-ray response to organonitrogen compound positive regulation of G2/M transition of mitotic cell cycle negative regulation of Wnt signaling pathway negative regulation of epithelial cell differentiation endoplasmic reticulum unfolded protein response mitotic G1 DNA damage checkpoint response to magnesium ion response to estradiol response to vitamin E Leydig cell differentiation mammary gland epithelial cell proliferation positive regulation of mammary gland epithelial cell proliferation response to drug response to estrogen response to leptin fat cell differentiation response to ethanol positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of cell cycle cell division response to corticosterone response to calcium ion canonical Wnt signaling pathway mammary gland alveolus development response to UV-A negative regulation of cell cycle arrest liver regeneration liver development positive regulation of cell proliferation response to organic substance response to organic cyclic compound Wnt signaling pathway organ regeneration regulation of protein kinase activity response to steroid hormone response to glucocorticoid regulation of cell cycle cellular response to organic substance regulation of G1/S transition of mitotic cell cycle regulation of transcription, DNA-templated cell cycle

Process(es)

transcription corepressor activity
protein kinase activity
protein binding
transcription factor binding
cyclin-dependent protein serine/threonine kinase regulator activity
enzyme binding
protein kinase binding
protein complex binding
histone deacetylase binding
proline-rich region binding
kinase activity

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Cyclin D1 Rabbit anti-Human, Mouse, Rat, Invitrogen

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Cyclin D1 Rabbit anti-Human, Mouse, Rat, Invitrogen

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Product Specific Information

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

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