Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA1-28870
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
ELISA (ELISA) | 1:5,000-1:50,000 |
Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent |
Immunohistochemistry (Paraffin) (IHC (P)) | 1:50-1:200 |
Immunoprecipitation (IP) | 1:100 |
Western Blot (WB) | 1:1,000-1:10,000 |
PUBLISHED APPLICATIONS | |
---|---|
Immunohistochemistry (IHC) | See 1 publications below |
Species reactivity | Bovine, Human |
Published species | Not Applicable |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Collagen Type III from human and bovine placenta |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1.16 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
Contains | 0.01% sodium azide |
Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID | AB_1956965 |
PA1-28870 has been successfully used in ELISA, immunohistochemistry (frozen tissue), immunoprecipitation, and Western blot applications. NOTE: The preparation of a specific antibody to Collagen III's uninterrupted "Glycine-X-Y" triplet repeat requires a non-denatured epitope format. Consequently, it is recommended to use this antibody in a Western blot using non-denaturing or "native" conditions.
Store as concentrated solution. Centrifuge briefly prior to opening vial.
Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in th gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alpha1 (III) collagen; COL 3; COL3; COL3 alpha 1; Collagen 3; Collagen alpha-1(III) chain; collagen, fetal; collagen, type III, alpha 1; collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant); collagen, type III, alpha 1 chain, fibril forming; Collagen3; Ehlers-Danlos syndrome type IV, autosomal dominant; FLJ34534; MGC93704
Gene Aliases: COL3A1; EDS4A
UniProt ID: (Human) P02461
Entrez Gene ID: (Bovine) 510833, (Human) 1281
Molecular Function:
extracellular matrix structural protein
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