Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA1-064
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunoprecipitation (IP) | 5 µg |
Western Blot (WB) | 1-2 µg/mL |
Immunocytochemistry (ICC/IF) | 1-2 µg/mL |
PUBLISHED APPLICATIONS | |
---|---|
Immunocytochemistry (ICC/IF) | See 4 publications below |
Western Blot (WB) | See 10 publications below |
Immunoprecipitation (IP) | See 1 publications below |
Immunohistochemistry (IHC) | See 1 publications below |
Species reactivity | Dog, Hamster, Human, Mouse, Rat |
Published species | Human, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptide corresponding to residues M(1) S G G K Y V D S E G H L Y T V P(17) C of human CAV1. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2072027 |
PA1-064 detects caveolin 1 from human, canine, hamster, mouse, and rat tissues and cells.
PA1-064 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects an ~22 kDa protein representing caveolin 1 from rat heart protein extract.
The PA1-064 immunogen is a synthetic peptide corresponding to residues M(1) S G G K Y V D S E G H L Y T V P(17) C of human CAV1. This sequence is completely conserved between human, mouse, canine, bovine, and rat. The PA1-064 immunizing peptide (Cat. # PEP-146) is available for use in neutralization and control experiments.
Caveolin-1 or CAV1 is a scaffolding proteins within caveolar membranes. It interacts directly with G-protein alpha subunits and can functionally regulate their activity. Caveolin-1 is also involved in the co-stimulatory signal essential for T-cell receptor (TCR) mediated T-cell activation. It can bind with DPP4 and induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Mutations affecting the CAV1 gene can result in congenital generalized lipodystrophy 3, pulmonary hypertension primary 3, or partial lipodystrophy/congenital cataracts, and neurodegeneration syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: caveolae; caveolin 1, caveolae protein, 22kDa; caveolin caveolae protein 22 kDa; caveolin, caveolae protein 1; caveolin, caveolae protein, 22 kDa; Caveolin-1; cell growth-inhibiting protein 32; HGNC:1527; OTTHUMP00000196475; Vesicular integral-membrane protein VIP21
Gene Aliases: BSCL3; CAV; Cav-1; CAV1; Caveolin-1; CGL3; LCCNS; MSTP085; PPH3; VIP21
UniProt ID: (Dog) P33724, (Human) Q03135, (Mouse) P49817
Entrez Gene ID: (Dog) 403980, (Human) 857, (Mouse) 12389, (Rat) 25404, (Chinese hamster) 100753021
Molecular Function:
scaffold/adaptor protein
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