Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA1-913
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Paraffin) (IHC (P)) | 1:100-1:500 |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1:1,000-1:10,000 |
Immunocytochemistry (ICC/IF) | Assay-dependent |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 130 publications below |
Immunohistochemistry (Paraffin) (IHC (P)) | See 1 publications below |
Immunohistochemistry (IHC) | See 5 publications below |
Miscellaneous PubMed (Misc) | See 2 publications below |
Immunoprecipitation (IP) | See 1 publications below |
Immunocytochemistry (ICC/IF) | See 3 publications below |
Species reactivity | Dog, Human, Mouse, Sheep, Rat |
Published species | Dog, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Purified canine cardiac calsequestrin. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | Conc. Not Determined |
Storage buffer | whole serum, PBS |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2071461 |
PA1-913 detects calsequestrin from canine, human, mouse, rat and sheep tissues. This antibody recognizes both cardiac and skeletal muscle calsequestrin.
PA1-913 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, immunohistochemistry (paraffin) and immunoprecipitation procedures. By Western blot, this antibody detects an ~55 kDa protein representing calsequestrin from canine cardiac extract. Additional bands at 97 kDa may be observed and have been reported to be calsequestrin-like proteins.
PA1-913 antigen is purified canine cardiac calsequestrin.
The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Aspartactin; calmitin; Calmitine; calsequestrin 1 (fast-twitch, skeletal muscle); Calsequestrin, skeletal muscle isoform; Calsequestrin-1; Laminin-binding protein
Gene Aliases: CASQ; CASQ1; CSQ; CSQ-1; CSQ1; PDIB1; sCSQ; VMCQA
UniProt ID: (Human) P31415, (Rat) P19633, (Mouse) O09165
Entrez Gene ID: (Sheep) 101118652, (Dog) 608401, (Human) 844, (Rat) 686019, (Mouse) 12372
Molecular Function:
calcium-binding protein
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.