Invitrogen
Catalog # PA5-50205
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Paraffin) (IHC (P)) | 1:50-1:200 |
Species reactivity | Mouse, Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Fusion protein corresponding to residues near the C terminal of human calpain 7 |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1.7 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS, pH 7.4, with 40% glycerol |
Contains | 0.05% sodium azide |
Storage conditions | -20°C |
RRID | AB_2635658 |
The antibody detects endogenous levels of total CAPN7 protein.
Calpain proteinases are defined currently as papain-like neutral proteases that are calcium activated. Calpains 1 and 2 are composed of a large subunit, which is proteolytically active, and a small subunit (also called calpain-4), or enhancer protein, that is not proteolytically active. The other calpain proteins identified to date (calpain 5, 6, 8, 9, 10, 11, 12, 13, 14, 15) are not known to require a small subunit. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV (domain-T in calpains 5 & 6, domain-N for calpain-7). Calpain-7 domain-N does not have the standard calpain EF hand domain for binding calcium, making it unclear whether it is calcium regulated. In place of the EF hand calcium-binding domain IV, calpain-7 has a sequence most closely resembling domain-III, with unknown function. Calpain-7 is widely distributed, found in most tissues analyzed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: calpain like protease; Calpain-7; homolog of Aspergillus Nidulans PALB; PalB homolog; PalBH
Gene Aliases: AU022319; CALPAIN7; CAPN7; PALBH
UniProt ID: (Human) Q9Y6W3, (Mouse) Q9R1S8
Entrez Gene ID: (Human) 23473, (Mouse) 12339
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