Invitrogen
Catalog # PA5-50385
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Western Blot (WB) | 1:500-1:2,000 |
Species reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Full length fusion protein of Human CPSF4 |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1.5 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS, pH 7.4, with 40% glycerol |
Contains | 0.05% sodium azide |
Storage conditions | -20°C |
RRID | AB_2635838 |
The antibody detects endogenous levels of total CPSF4 protein.
Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cleavage and polyadenylation specific factor 4, 30kDa; Cleavage and polyadenylation specificity factor 30 kDa subunit; Cleavage and polyadenylation specificity factor subunit 4; CPSF 30 kDa subunit; Neb-1; no; No arches homolog; no arches-like zinc finger protein; NS1 effector domain-binding protein 1
Gene Aliases: CPSF30; CPSF4; NAR; NEB-1; NEB1
UniProt ID: (Human) O95639
Entrez Gene ID: (Human) 10898
Molecular Function:
RNA splicing factor
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