TESTED APPLICATIONS | DILUTION |
---|---|
Western Blot (WB) | 1 µg/mL |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 1 publications below |
Published species | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptide corresponding to residues C(287) D V Q T R V V A A G S L(299) of bovine GARP2. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2260720 |
PA1-728 detects glutamic acid rich protein2 (GARP2) protein in rat and bovine samples.
PA1-728 has successfully been used in Western blot procedures. By Western blot, this antibody detects an ~60 kDa protein representing GARP2 from rat purified rod outer membranes.
The PA1-728 immunogen is a synthetic peptide corresponding to residues C(287) D V Q T R V V A A G S L(299) of bovine GARP2. This immunizing peptide (PEP-247) can be used for neutralization and control experiments.
Glutamic acid rich protein (GARP) is a soluble protein localized to the outer segments of the rod photoreceptor. GARPs are characterized by six repeated motifs of: V V E K K N/E E. These repeat motifs help form an alpha-helix structure. The function(s) of GARP have not been fully characterized, but studies have suggested that GARP helps limit cGMP turnover by inhibiting phosphodiesterase activity. To date, there have been two splice variants characterized: GARP1 (full-length GARP) and GARP2 (truncated GARP). GARP also interacts with part of the larger cytoplasmic domain of the beta-subunit of the cyclic GMP-gated channel found in the plasma membrane.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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