Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA1-4316
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Western Blot (WB) | 1:500-1:1,000 |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 1 publications below |
Species reactivity | Human, Mouse |
Published species | Not Applicable |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptide corresponding to residues L(10) L Y P K S L S R H V S V R T S(25) of human CIDE-3. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS |
Contains | 0.02% sodium azide |
Storage conditions | Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C |
RRID | AB_2079998 |
PA1-4316 detects the human CIDE-3 protein in human samples. Expected to recognize mouse samples due to 93% sequence homology.
PA1-4316 has been successfully used in Western blot procedures. By Western blot this antibody detects a ~27 kDa protein representing human CIDE-3 in HepG2 cell lysate.
The PA1-4316 immunogen is a synthetic peptide corresponding to residues L(10) L Y P K S L S R H V S V R T S(25) of human CIDE-3. This antibody shows cross-reactivity to FSP27, which is the mouse homolog of CIDE-3, with a sequence identity of 93%.
Maintain at 4ºC for 3 months or at -20ºC for longer periods. Avoid repeated freeze-thaw cycles.
DNA fragmentation is one of the critical steps in apoptosis, which is induced by DNA fragmentation factor (DFF). DFF is composed of two subunits, a 40 kDa caspase-activated nuclease (DFF40) and a 45 kDa inhibitor (DFF45). Recently a novel family of cell-death-inducing DFF45-like effectors (CIDEs) has been identified. Human CIDE-3 is a novel member of CIDEs. There are 2 transcripts, CIDE-3 and CIDE-3alpha, were present in HepG2 and A375 cells. Consistent with its chromosome localization at 3p25, a region associated with high frequency loss of heterozygosity in many tumours, CIDE-3 may play an important role in prevention of tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cell death activator CIDE-3; cell death-inducing DFFA-like effector c; Cell death-inducing DFFA-like effector protein C; CID; cide 3; CIDE-3alpha; fat specific gene 27; fat specific protein 27; Fat-specific protein FSP27; Fat-specific protein FSP27 homolog; Lipid transferase CIDEC
Gene Aliases: CIDE-3; CIDE3; CIDEC; FPLD5; FSP27
UniProt ID: (Human) Q96AQ7, (Mouse) P56198
Entrez Gene ID: (Human) 63924, (Mouse) 14311
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