Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # MA1-250
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Frozen) (IHC (F)) | 1/100 |
Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1 µg/mL |
Immunocytochemistry (ICC/IF) | Assay-dependent |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 71 publications below |
Immunocytochemistry (ICC/IF) | See 8 publications below |
Immunohistochemistry (IHC) | See 8 publications below |
Immunohistochemistry (Paraffin) (IHC (P)) | See 1 publications below |
Miscellaneous PubMed (Misc) | See 3 publications below |
Species reactivity | Human, Mouse |
Published species | Human, Mouse, Rabbit, Rat, Sheep |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Clone | 9C8 |
Immunogen | A bacterially expressed, mouse CHOP fusion protein. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Protein A |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2292611 |
MA1-250 detects CHOP protein in human and mouse samples.
MA1-250 has successfully been used in Western blot, immunoprecipitation, immunocytochemistry, and immunofluorescence procedures. By Western blot, this antibody detects an ~31 kDa protein representing CHOP from primary human fibroblast extract.
The MA1-250 immunizing protein corresponds to a bacterially expressed, mouse CHOP fusion protein. Clone 9C8 has been shown to recognize an epitope in the N-terminal region of CHOP.
This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
GADD153 is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP GADD153 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C/EBP homoologous protein 10; C/EBP zeta; C/EBP-homologous protein; C/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; CHOP; CHOP-10; DDIT-3; DNA damage-inducible transcript 3 protein; DNA-damage-inducible transcript 3; Growth arrest and DNA damage-inducible protein GADD153; Growth arrest and DNA-damage-inducible protein GADD153; MGC4154
Gene Aliases: CEBPZ; CHOP; CHOP-10; CHOP10; DDIT3; GADD153
UniProt ID: (Human) P35638, (Mouse) P35639
Entrez Gene ID: (Human) 1649, (Mouse) 13198
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