Invitrogen
IP-mass spectrometry View details.
Catalog # AHZ0202
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Flow Cytometry (Flow) | Assay-Dependent |
Immunoprecipitation (IP) | Assay-Dependent |
Western Blot (WB) | Assay-Dependent |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 10 publications below |
Immunohistochemistry (IHC) | See 16 publications below |
Immunocytochemistry (ICC/IF) | See 2 publications below |
Immunohistochemistry (Paraffin) (IHC (P)) | See 6 publications below |
Miscellaneous PubMed (Misc) | See 11 publications below |
Species reactivity | Human, Mouse, Rat |
Published species | Human, Rabbit, Tag |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Clone | DCS-31 |
Immunogen | Purified recombinant cdk4 protein. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 0.2 mg/mL |
Purification | Protein G |
Storage buffer | PBS, pH 7.4, with 0.2% BSA |
Contains | 0.09% sodium azide |
Storage conditions | 4° C |
RRID | AB_2536376 |
This antibody reacts with an epitope at aa 16-35.
Recommended positive controls include MAD109, LS174T, and PC12 cells.
CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cell division protein kinase 4; CRK3; cyclin dependent kinase 4; Cyclin-dependent kinase 4; MGC14458; p34<PSK-J3>/cdk4; PSK-J3; serine/threonine kinase
Gene Aliases: CDK4; CMM3; Crk3; PSK-J3
UniProt ID: (Human) P11802, (Rat) P35426, (Mouse) P30285
Entrez Gene ID: (Human) 1019, (Rat) 94201, (Mouse) 12567
Molecular Function: non-receptor serine/threonine protein kinase
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