Catalog # PA1-847
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
ChIP assay (ChIP) | 1-3 µL |
Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000-1:5,000 |
Western Blot (WB) | 1-2 µg/mL |
Immunocytochemistry (ICC/IF) | 1:50-1:500 |
PUBLISHED APPLICATIONS | |
---|---|
ChIP assay (ChIP) | See 1 publications below |
Western Blot (WB) | See 8 publications below |
Immunohistochemistry (IHC) | See 2 publications below |
Immunocytochemistry (ICC/IF) | See 1 publications below |
Species reactivity | Human, Mouse |
Published species | Human, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptide corresponding to residues A(162) T S S P A T S Q T G P G I C(176) in the nuclear factor binding domain of human CBP. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2083939 |
PA1-847 detects cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) from human and mouse tissues and cells.
PA1-847 has been successfully used in ChIP, ICC/IF, Western blot and IHC-P procedures. By Western blot, this antibody detects an ~265 kDa protein representing CBP and a larger, unknown protein at ~400 kDa from HeLa cell lysate.
The PA1-847 immunogen is a synthetic peptide corresponding to residues A(162) T S S P A T S Q T G P G I C(176) in the nuclear factor binding domain of human CBP. The PA1-847 immunizing peptide (Cat. # PEP-052) is available for use in neutralization and control experiments.
CBP for CREB-binding protein is a 265 kDa nuclear protein which probably has many of the properties expected of a CREB co-activator. It shows substantial sequence homology with p300.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CREB-binding protein; Histone lysine acetyltransferase CREBBP; Protein-lysine acetyltransferase CREBBP
Gene Aliases: AW558298; CBP; CBP/p300; CREBBP; KAT3A; p300/CBP; RSTS
UniProt ID: (Human) Q92793
Entrez Gene ID: (Human) 1387, (Mouse) 12914
Molecular Function:
histone modifying enzyme
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