Invitrogen
Catalog # PA1-544
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (IHC) | Assay-dependent |
Western Blot (WB) | 0.5-2 µg/mL |
Immunocytochemistry (ICC/IF) | 2 µg/mL |
Species reactivity | Human, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic Peptide: C(578) E R D S P S T S R Q S P A N(592) |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2243867 |
PA1-544 detects calmodulin sensitive kinase (CASK) from mouse and rat tissues and cells.
PA1-544 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects a 112 kDa protein representing CASK from AtT20 cell lysate. Immunohistochemical staining of CASK in mouse brain with PA1-544 results in staining of the choroid plexus.
PA1-544 immunizing peptide corresponds to amino acid residues 578-592 from human CASK. This sequence is completely conserved between rat, mouse, and human CASK.
PA1-544 can be used with blocking peptide PEP-110.
CASK is a calcium/calmodulin-dependent serine protein kinase which is a MAGUK (membrane-associated guanylate kinase) protein family member. CASK is a scaffold protein and the encoded protein is located at synapses in the brain. CASK associates with FG syndrome 4, mental retardation, microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked mental retardation. CASK functions as a cytoskeletal membrane scaffold that coordinates signal transduction pathways within the cortical cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: calcium/calmodulin-dependent serin protein kinase; Calcium/calmodulin-dependent serine protein kinase; calcium/calmodulin-dependent serine protein kinase (MAGUK family); calcium/calmodulin-dependent serine protein kinase membrane-associated guanylate kinase; hCASK; MGC7449; OTTHUMP00000023157; OTTHUMP00000023161; OTTHUMP00000023162; Peripheral plasma membrane protein CASK; Protein lin-2 homolog; RP23-278L4.1; trinucleotide repeat containing 8
Gene Aliases: CAGH39; CAMGUK; CASK; CMG; DXPri1; DXRib1; FGS4; LIN-2; LIN2; MICPCH; mLin-2; MRXSNA; Pals3; TNRC8
UniProt ID: (Human) O14936, (Rat) Q62915, (Mouse) O70589
Entrez Gene ID: (Human) 8573, (Rat) 29647, (Mouse) 12361
Molecular Function:
scaffold/adaptor protein
non-receptor serine/threonine protein kinase
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