Catalog # MA3-921
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Flow Cytometry (Flow) | 1:100-1:200 |
Immunohistochemistry (Frozen) (IHC (F)) | 1:500 |
Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
Western Blot (WB) | 1:500-1:1,000 |
Immunocytochemistry (ICC/IF) | 1:250 |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 34 publications below |
Immunohistochemistry (IHC) | See 5 publications below |
Flow Cytometry (Flow) | See 2 publications below |
Immunocytochemistry (ICC/IF) | See 5 publications below |
Species reactivity | Guinea pig, Human, Mouse, Rabbit, Rat |
Published species | Fish, Human, Mouse, Pig, Rabbit, Rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Clone | 20A |
Immunogen | Purified rabbit dihydropyridine receptor. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | Conc. Not Determined |
Storage buffer | ascites |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2069904 |
MA3-921 detects 1,4-dihydropyridine (DHP) receptor alpha-2 subunit from human, rat, mouse, guinea pig and rabbit skeletal and cardiac muscle.
MA3-921 has been successfully used in Western blot, immunocytochemistry, immunofluorescence, and immunohistochemistry procedures. By Western blot, this antibody detects a 220 kDa protein under non-reducing and a 143 kDa protein under reducing conditions representing DHP from rabbit skeletal muscle membrane preparations. Immunohistochemical staining of DHP in rabbit skeletal muscle with MA3-921 results in double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines.
The MA3-921 antigen is purified rabbit DHP receptor.
This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Calcium channel subunit alpha 2 delta (dihydropyridine - sensitive L-type); calcium channel, L type, alpha 2 polypeptide; calcium channel, voltage-dependent, alpha 2/delta subunit 1; dihydropryridine-sensitive calcium channel alpha-2 subunit; dihydropyridine-sensitive calcium channel alpha 2; dihydropyridine-sensitive L-type, calcium channel alpha-2/delta subunit; H_DJ0560O14.1; Voltage-dependent calcium channel subunit alpha-2/delta-1; Voltage-gated calcium channel subunit alpha-2/delta-1; WUGSC:H_DJ0560O14.1
Gene Aliases: Ca(v)alpha2delta1; CACNA2; CACNA2D1; CACNL2A; CCHL2A; CCHLA2; DHSCCA; LINC01112; lncRNA-N3; MHS3
UniProt ID: (Rabbit) P13806, (Human) P54289, (Mouse) O08532
Entrez Gene ID: (Rabbit) 100009105, (Human) 781, (Rat) 25399, (Mouse) 12293
Molecular Function:
voltage-gated ion channel
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