Invitrogen
Catalog # PA5-49593
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Flow Cytometry (Flow) | 1:10-1:50 |
Immunohistochemistry (Paraffin) (IHC (P)) | 1:50-1:100 |
Western Blot (WB) | 1:1,000 |
Species reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | KLH conjugated synthetic peptide between 362-390 amino acids from the C-terminal region of human BCKDHA. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1.4 mg/mL |
Purification | Ammonium sulfate precipitation |
Storage buffer | PBS, pH 7.4 |
Contains | 0.09% sodium azide |
Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID | AB_2635047 |
The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of the branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine. This reaction is the second major step in the catabolism of the branched-chain amino acids (Wynn et al., 1998). The BCKD complex consists of 3 catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase (E2), and a homodimeric dihydrolipoamide dehydrogenase (E3). E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene encodes the beta subunit of E1. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 2-oxoisovalerate dehydrogenase (lipoamide); 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; BCKDE1A; BCKDH E1-alpha; branched chain keto acid dehydrogenase E1 alpha protein; Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain
Gene Aliases: BCKDE1A; BCKDHA; MSU; MSUD1; OVD1A
UniProt ID: (Human) P12694
Entrez Gene ID: (Human) 593
Molecular Function:
dehydrogenase
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