Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA1-757
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
ELISA (ELISA) | Assay-dependent |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1:200-1:2,000 |
Immunocytochemistry (ICC/IF) | 1:20-1:200 |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 28 publications below |
Immunocytochemistry (ICC/IF) | See 5 publications below |
Immunoprecipitation (IP) | See 5 publications below |
ELISA (ELISA) | See 1 publications below |
Species reactivity | Human, Mouse |
Published species | Human, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptide corresponding to residues C(485) L R Q Q H D D F A D D I S L L K(501) of human beta-secretase. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_325863 |
PA1-757 detects transfected beta-secretase 1.
PA1-757 has been successfully used in ELISA, ICC/IF, Western blot and immunoprecipitation applications. The molecular weight of BACE1 detected may vary depending on the lysate tested. This may be due to glycosylation differences in various cell lines.
The PA1-757 immunizing peptide (Cat. # PEP-093) is available for use in neutralization and control experiments.
BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides (Abeta) found in extracellular amyloid plaques of Alzheimer's disease (AD). In some cases early onset familial AD can be attributed to a "Swedish" mutation in the amyloid precursor protein (APP), which dramatically enhances the cleavage of this protein by BACE1. This and other genetic and pathological evidence has led to therapeutic approaches that have focused on the inhibition of BACE1 and other APP-cleaving enzymes, such as gamma-secretase stuff. APP is first cleaved by b-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-b peptide, which is deposited in the brains of all sufferers of Alzheimer's disease. The long-sought b-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). bACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: APP beta-secretase; asp 2; ASP2; Aspartyl protease 2; BACE 1; BACE-1D; BACE-I-432; Beta secretase; Beta-secretase 1; beta-secretase 1 precursor variant 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme 1; Memapsin; Memapsin-2; Memapsin2; Membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2
Gene Aliases: ASP2; BACE; BACE1; C76936; HSPC104; KIAA1149
UniProt ID: (Human) P56817, (Mouse) P56818
Entrez Gene ID: (Human) 23621, (Mouse) 23821
Molecular Function:
protease
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