Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # MA1-054
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
ELISA (ELISA) | Assay-dependent |
Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
Western Blot (WB) | 2 µg/mL |
Immunocytochemistry (ICC/IF) | 2 µg/mL |
PUBLISHED APPLICATIONS | |
---|---|
Immunohistochemistry (PFA fixed) (IHC (PFA)) | See 1 publications below |
Immunocytochemistry (ICC/IF) | See 2 publications below |
Western Blot (WB) | See 14 publications below |
Immunohistochemistry (IHC) | See 4 publications below |
Immunohistochemistry (Paraffin) (IHC (P)) | See 1 publications below |
ELISA (ELISA) | See 1 publications below |
Miscellaneous PubMed (Misc) | See 2 publications below |
Species reactivity | Human, Mouse, Rabbit |
Published species | Bovine, Human, Mouse, Rabbit |
Host / Isotype | Mouse / IgG |
Class | Monoclonal |
Type | Antibody |
Clone | 19F1 |
Immunogen | Full length, recombinant, human adiponectin. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Protein G |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_557516 |
MA1-054 detects adiponectin from human, rabbit and mouse samples.
MA1-054 has been used successfully in Western blot, immunofluorescence, and ELISA procedures. By Western blot this antibody detects ~30 kDa protein representing Adiponectin in 3T3-L1 adipocytes. Immunofluorescence and ELISA of Adiponectin can also be performed using MA1-054.
The MA1-054 immunogen is full length, recombinant, human adiponectin.
Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates the metabolism of lipids and glucose. Adiponectin has been shown to play a role in various physiological processes including homeostasis and obesity. Decreased levels of adiponectin are associated with obese individuals as well as insulin resistance and hyperinsulinemia (type 2 diabetes). In mice adiponectin has been shown to improve insulin signaling, thus ameliorating insulin resistance. Through activation of AMP kinase and PPAR-alpha, adiponectin can increase fatty acid transport, oxidation, and dissipation in skeletal muscle, leading to a reduction of intramyocellular lipid levels and thus improved insulin signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 30 kDa adipocyte complement-related protein; ACRP30; adipocyte complement related protein; Adipocyte complement-related 30 kDa protein; adipocyte complement-related protein of 30 kDa; adipocyte, C1Q and collagen domain containing; Adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ; Adiponectin; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; ADPIOQ; apM-1; Gelatin-binding protein; gelatin-binding protein 28
Gene Aliases: 30kDa; ACDC; ACRP30; Ad; adipo; ADIPOQ; ADIPQTL1; ADPN; APM-1; APM1; APN; GBP28
UniProt ID: (Human) Q15848, (Mouse) Q60994
Entrez Gene ID: (Human) 9370, (Rabbit) 100009027, (Mouse) 11450
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